Evidence for acetylated amino-terminal residues in hemocyanins.
Evidence for acetylated amino-terminal residues in hemocyanins.
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发表时间：
1981-10
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通讯作者：
P. Zatta;B. Salvato
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作者：
P. Zatta;B. Salvato
用氰酸法和丹磺酰氯法测定Macinus maenas血蓝蛋白的NH2氨基酸末端，得率很低。这给出了比物理方法测定的分子量高得不寻常的值。假设NH2末端是乙酰化的，则乙酰基的数量(用荧光分光光度法确定)占存在于血蓝蛋白中的所有氨基糖和NH2末端的数量，假设多肽链的分子量为25,000道尔顿。
The NH2 amino acid terminals of hemocyanin from Carcinus maenas determined by the cyanate and by the dansyl-chloride methods gave very low yields. This gave values for the molecular weight which were abnormally high compared to those determined by physical methods. Assuming that the NH2 terminals are acetylated, the number of acetyl groups (determined spectrofluorimetrically) accounts for all the amino sugars present in hemocyanin and for the NH2 terminals assuming that the molecular weight of the polypeptide chains was 25,000 daltons.