DEVELOPMENT OF DIENCEPHALON IN RAT .1. AUTORADIOGRAPHIC STUDY OF TIME OF ORIGIN AND SETTLING PATTERNS OF NEURONS OF HYPOTHALAMUS
DEVELOPMENT OF DIENCEPHALON IN RAT .1. AUTORADIOGRAPHIC STUDY OF TIME OF ORIGIN AND SETTLING PATTERNS OF NEURONS OF HYPOTHALAMUS
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DOI：
10.1002/cne.901820511
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发表时间：
1978-01-01
影响因子：
2.5
通讯作者：
BAYER, SA
中科院分区：
文献类型：
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作者：
ALTMAN, J;BAYER, SA
从妊娠第[E] 13天（E13 + 14）至出生前一天（E21 + 22），对各组妊娠大鼠连续注射2次每日剂量的3 H-胸苷。这种双标记程序与组间延迟一天的注射时间表相结合（E13 + 14; E14 + 15; E15 + 16..）。这2次注射确保了在分化开始之前对给定结构的几乎所有神经元进行全面标记（全面标记），而每天注射的渐进延迟使得有可能估计一天内下丘脑不同区域中形成的神经元的比例。根据细胞遗传学等时性，将下丘脑各核团分为4类。由最早出现的（1类）神经元组成的结构构成了外侧层，包括外侧视前区和外侧下丘脑区以及外侧乳头核。由早期出现的（2类）神经元组成的结构形成了一个异质的核系统，包括室旁核、核间核和视上大细胞神经元以及下丘脑前部和后部的几个中间层核。晚发核（第3类）和最晚发核（第4类）在前部构成一个室周系统，在后部构成一个更广泛的区域。后两个核系统可能构成下丘脑的促垂体区。乳头体系统的核是顺序产生的，与下丘脑的其他结构不同，它们的产生时间更快。核间标记梯度用于推断神经上皮的起源和神经元的沉降模式。共同的网站的起源表示为大细胞神经垂体神经元，背内侧和腹内侧核的神经元和结节乳头和弓状核的神经元。这些核团的起源地点与成熟下丘脑中专门的心室衬里有关。
Groups of pregnant rats were injected with 2 successive daily doses of 3H-thymidine from gestational day [E] 13 (E13 + 14) until the day before birth (E21 + 22). This double labeling procedure was combined with an injection schedule of a single day delay between groups (E13 + 14; E14 + 15; E15 + 16..). The 2 injections assured the comprehensive labeling of practically all neurons of a given structure prior to the onset of their differentiation (comprehensive labeling) whereas the progressive daily delay in injections made it possible to estimate the proportion of neurons formed in various regions of the hypothalamus on a single day. Hypothalamic areas or nuclei were assigned into 4 classes on the basis of their cytogenetic isochronicity. Structures composed of the earliest arising (class 1) neurons constitute a lateral tier that includes the lateral preoptic and lateral hypothalamic areas, and the lateral mammillary nucleus. Structures composed of early arising (class 2) neurons form a heterogeneous collection of nuclear systems including the paraventricular, internuclear and supraoptic magnocellular neurons and several intermediate tier nuclei of the anterior and posterior hypothalamus. The late arising (class 3) and latest arising (class 4) nuclei constitute a periventricular system anteriorly and a more extensive region posteriorly. The latter 2 nuclear systems may constitute the hypophysiotropic area of the hypothalamus. The nuclei of the mammillary system, which are produced sequentially, are distinguished from other hypothalamic structures by their more rapid generation time. Internuclear labeling gradients were used to infer the neuroepithelial site of origin and settling pattern of neurons. Common sites of origin were indicated for the magnocellular neurohypophysial neurons, the neurons of the dorsomedial and ventromedial nuclei and the neurons of the tuberomammillary and arcuate nuclei. The sites of origin of these groups of nuclei were related to specialized ventricular linings in the mature hypothalamus.