AtSTP6, a New Pollen-Specific H+-Monosaccharide Symporter from Arabidopsis1
AtSTP6, a New Pollen-Specific H+-Monosaccharide Symporter from Arabidopsis1
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DOI：
10.1104/pp.012666
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发表时间：
2003-01
期刊：
影响因子：
7.4
通讯作者：
J. Scholz‐Starke;M. Büttner;N. Sauer
中科院分区：
文献类型：
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作者：
J. Scholz‐Starke;M. Büttner;N. Sauer
本文描述了拟南芥 H+/单糖转运蛋白家族的新成员 AtSTP6 的分子、动力学和生理学特征。 AtSTP6 基因 (At3g05960) 被两个内含子打断，编码含有 12 个假定跨膜螺旋的 507 个氨基酸的蛋白质。在酵母（酿酒酵母）中的表达表明，AtSTP6 是一种高亲和力 (K m = 20 μm)、广谱、解偶联剂敏感的单糖转运蛋白，靶向质膜，可以补充大多数酵母己糖转运蛋白基因破坏导致的生长缺陷。 AtSTP6启动子::GUS植物的分析和原位杂交实验仅在花粉发育的后期检测到AtSTP6表达。分离了转座子标记的拟南芥突变体，并分析了纯合植物 Atstp6 突变对花粉活力、花粉萌发、受精和种子生产的潜在影响。然而，无法观察到野生型和突变体植物之间的差异。
This paper describes the molecular, kinetic, and physiological characterization of AtSTP6, a new member of the Arabidopsis H+/monosaccharide transporter family. The AtSTP6 gene (At3g05960) is interrupted by two introns and encodes a protein of 507 amino acids containing 12 putative transmembrane helices. Expression in yeast (Saccharomyces cerevisiae) shows that AtSTP6 is a high-affinity (K m = 20 μm), broad-spectrum, and uncoupler-sensitive monosaccharide transporter that is targeted to the plasma membrane and that can complement a growth deficiency resulting from the disruption of most yeast hexose transporter genes. Analyses ofAtSTP6-promoter::GUS plants and in situ hybridization experiments detected AtSTP6expression only during the late stages of pollen development. A transposon-tagged Arabidopsis mutant was isolated and homozygous plants were analyzed for potential effects of the Atstp6mutation on pollen viability, pollen germination, fertilization, and seed production. However, differences between wild-type and mutant plants could not be observed.