Fluorescently labeled mesenchymal stem cells (MSCs) maintain multilineage potential and can be detected following implantation into articular cartilage defects
Fluorescently labeled mesenchymal stem cells (MSCs) maintain multilineage potential and can be detected following implantation into articular cartilage defects
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DOI：
10.1016/s0142-9612(01)00086-2
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发表时间：
2002-01-01
期刊：
影响因子：
14
通讯作者：
Pellas, TC
中科院分区：
文献类型：
--
作者：
Quintavalla, J;Uziel-Fusi, S;Pellas, TC
几项研究已经报道了在将间充质干细胞（MSC）植入全层软骨缺损中后受损软骨的修复增强，这表明修复组织中的细胞来源于植入物。然而，不能排除增强的组织修复来源于响应于植入物而募集到缺损的宿主细胞，而不是通过植入的MSC重新增殖组织。我们的目的是研究荧光标记的MSC在体内植入全层软骨缺损后的短期命运，我们研究中使用的荧光染料并不影响MSC的活力或它们在体外进行成骨和成软骨分化的能力。将MSC/明胶结构植入山羊的全层软骨缺损中。这些细胞保留染料，并可通过组织学和流式细胞术检测。在植入后2周的时间间隔内，我们观察到植入细胞在缺损中逐渐丢失，以及含有标记的骨髓间充质干细胞的明胶海绵碎片在骨髓深部空间中指示碎片，移位和被动migration.Fluorescent标记使我们能够确定植入细胞是否在植入后的早期时间点丢失以及它们在整个缺损中的空间取向。通过确定植入细胞的命运，可以设计新的生物材料来纠正不良特性。在短期体内模型中测试新的生物材料将为成功的长期软骨再生所需的细胞保留提供更快的优化。(C)2001爱思唯尔科技有限公司版权所有。
Several studies have reported enhanced repair of damaged cartilage following implantation of mesenchymal stem cells (MSCs) into full-thickness cartilage defects suggesting that the cells in the repair tissue were derived from the implant. However, it cannot be excluded that the enhanced tissue repair is derived from host cells recruited to the defect in response to the implant, rather than the re-population of the tissue by the implanted MSCs. Our objective was to study the short-term fate of fluorescently labeled MSCs after implantation into full-thickness cartilage defects in vivo.The fluorescent dye used in our studies did not affect MSC viability or their ability to undergo osteogenic and chondrogenic differentiation in vitro. MSC/gelatin constructs were implanted into full-thickness cartilage defects in goats. These cells retained the dye and were detectable by histology and flow cytometry. At intervals spanning 2 weeks post-implantation we observed gradual loss of implanted cells in the defect as well as fragments of gelatin sponge containing labeled MSCs in deep marrow spaces indicating fragmentation, dislodgement and passive migration.Fluorescent labeling enabled us to determine whether the implanted cells were lost during early time points after implantation as well as their spatial orientation throughout the defect. By determining the fate of implanted cells, new biomaterials could be engineered to correct undesirable characteristics. Testing of new biomaterials in short-term in vivo models would provide faster optimization for cell retention needed for successful, long-term cartilage regeneration. (C) 2001 Elsevier Science Ltd. All rights reserved.