IMMUNOGOLD FOR DETECTION OF ANTIGEN ON NITROCELLULOSE PAPER
IMMUNOGOLD FOR DETECTION OF ANTIGEN ON NITROCELLULOSE PAPER
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DOI：
10.1016/0003-2697(84)90542-6
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发表时间：
1984-01-01
影响因子：
2.9
通讯作者：
HSU, YH
中科院分区：
文献类型：
--
作者：
HSU, YH
介绍了免疫金在硝酸纤维素纸抗原抗体复合体检测中的应用。将纯化的烟草花叶病毒(TMV)蛋白或叶提取物与硝酸纤维素纸结合后，与兔抗TMV血清作用。用金标记的羊抗兔抗体检测抗原-抗体复合体。金标抗体是直接可见的，因为它是粉红色的。该方法可以检测到1-5pg的烟草花叶病毒蛋白，无论是纯化后的TMV蛋白还是未纯化的植物提取物中的TMV蛋白，都具有很高的特异性。
The application of immunogold for the detection of antigen-antibody complexes on nitrocellulose paper is described. Tobacco mosaic virus (TMV) protein, either purified or in leaf extract, was bound to the nitrocellulose paper and then exposed to rabbit anti-TMV serum. The antigen-antibody complexes were detected by gold-labeled goat anti-rabbit IgG. The gold-labeled antibody is directly visible because of its pink color. This method can detect 1-5 pg of TMV protein, either in purified form or in the unpurified plant extract, with high specificity.