Liver reconstruction on the chorioallantoic membrane of the chick embryo
Liver reconstruction on the chorioallantoic membrane of the chick embryo
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DOI：
10.1679/aohc.73.45
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发表时间：
2010-01-01
影响因子：
--
通讯作者：
Hirano, Shigeki
中科院分区：
文献类型：
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作者：
Chiba, Akina;Yui, Chisato;Hirano, Shigeki
将6日龄鸡胚的肝脏移植到9日龄鸡胚的绒毛膜尿囊膜上，观察肝脏再生的组织学过程。当一块肝脏被移植到绒毛膜尿囊膜上时，只有附着在绒毛膜尿囊膜上的移植物的浅层细胞存活。最终，这些存活在浅表区的细胞随着造血增生而增殖，形成被肝细胞（或肝实质细胞）包围的血细胞簇。半薄连续切片显示，这些血细胞团被限制在肝细胞形成的空间内。此外，移植后11天重建肝脏中出现了类似肝索、肝窦、中央静脉和胆管的结构。同时，将游离的肝细胞小球移植到绒毛膜尿囊膜上，重建了两种肝样结构：一种是造血后的肝细胞簇状并伴有窦状结构，另一种是单纯的肝细胞堆积，没有任何窦状结构和造血。在前一种类型中发现的窦随着移植空间与绒毛膜尿囊膜血管的连接而变得清晰。这些结果表明，重建肝脏主要是由肝细胞聚集并伴有造血细胞形成，然后形成窦腔。因此，我们认为造血对于正常结构的肝脏再生是重要的。肝移植在绒毛膜尿囊膜内也可用于肝再生的研究。
The liver from a 6-day-old chick embryo was transplanted on the chorioallantoic membrane of a 9-day-old chick embryo to observe the process of liver regeneration histologically. When a piece of the liver was implanted on the chorioallantoic membrane, only cells in the superficial zone of the graft adhering to the chorioallantoic membrane survived. Eventually, these surviving cells in the superficial zone proliferated with hematopoiesis, resulting in the formation of clusters of blood cells surrounded by the hepatocytes (or hepatic parenchymal cells). Semi-thin serial sections showed that these clusters of blood cells were confined to the space formed by hepatocytes. Furthermore, structures similar to the hepatic cord, sinusoid, central vein, and bile duct appeared in the reconstructed liver eleven days after transplantation. Meanwhile, when a pellet of the dissociated liver cells was transplanted onto the chorioallantoic membrane, two types of liver like structures were reconstructed: one was clusters of hepatocytes accompanied by sinusoids after hematopoiesis, and the other was a simple accumulation of hepatocytes without any sinusoids or hematopoiesis. The sinusoids found in the former type became clear following the connection between the space in the transplant and vessels of the chorioallantoic membrane. These findings indicated that the reconstructed liver was primarily produced by the accumulation of hepatocytes accompanied by hematopoietic cells, followed by the formation of sinusoidal spaces. We therefore consider that hematopoiesis is important for liver regeneration with a normal structure. Transplantation of the liver in the chorioallantoic membrane could be also useful for research into liver regeneration.