Determination of individual vitamin B6 compounds based on enzymatic conversion to 4-pyridoxolactone
Determination of individual vitamin B6 compounds based on enzymatic conversion to 4-pyridoxolactone
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DOI：
10.3177/jnsv.54.18
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发表时间：
2008-02-01
影响因子：
1.6
通讯作者：
Yagi, Toshiharu
中科院分区：
文献类型：
--
作者：
Nishimura, Saki;Nagano, Sayaka;Yagi, Toshiharu
建立了天然维生素136单体化合物的测定方法。维生素136化合物通过酶促反应和HCl水解的组合被特异性地转化为4-吡哆内酯（PAL），一种高荧光化合物。然后通过HPLC测定PAL。吡哆醛4-脱氢酶（PLDH）可将吡哆醛完全氧化为PAL。吡哆醇和吡哆胺分别通过吡哆醇4-氧化酶和吡哆胺-丙酮酸氨基转移酶与PLDH的偶联反应完全转化为PAL。用HCl水解5 '-磷酸盐形式和吡哆醇-β-葡萄糖苷，然后测定其游离形式。此处未单独测定吡哆醇5 '-磷酸和吡哆醇-β-葡萄糖苷。用此方法分析了三种食品样品。
A determination method for individual natural vitamin 136 compounds was developed. The vitamin 136 compounds were specifically converted into 4-pyridoxolactone (PAL), a highly fluorescent compound, through a combination of enzymatic reactions and HCI-hydrolysis. PAL was then determined by HPLC. Pyridoxal was completely oxidized to PAL with pyridoxal 4-dehydrogenase (PLDH). Pyridoxine and pyridoxamine were totally converted into PAL through a coupling reaction involving pyridoxine 4-oxidase and PLDH, and one involving pyridoxamine-pyruvate aminotransferase and PLDH, respectively. The 5'-phosphate forms and pyridoxine-beta-glucoside were hydrolyzed with HCl, and then determined as their free forms. Pyridoxine 5'-phosphate and pyridoxine-beta-glucoside were not separately determined here. Three food samples were analyzed by this method.