cAMP-dependent protein kinase A in grass carp Ctenopharyngodon idella: Molecular characterization, gene structure, tissue distribution and mRNA expression in endoplasmic reticulum stress-induced adipocyte lipolysis
cAMP-dependent protein kinase A in grass carp Ctenopharyngodon idella: Molecular characterization, gene structure, tissue distribution and mRNA expression in endoplasmic reticulum stress-induced adipocyte lipolysis
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草鱼Ctenopharyngodon idella中cAMP依赖性蛋白激酶A：内质网应激诱导脂肪细胞脂肪分解的分子特征、基因结构、组织分布和mRNA表达
DOI：
10.1016/j.cbpb.2020.110479
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发表时间：
2020-12-01
影响因子：
2.2
通讯作者：
Ji, Hong
中科院分区：
文献类型：
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作者：
Ji, Shanghong;Sun, Jian;Ji, Hong
蛋白激酶A（PKA）是研究最多的蛋白激酶之一，在细胞中具有多种功能，包括调节糖和脂质的代谢。本研究鉴定了草鱼PKA家族的9种亚型，并获得了它们的完整编码序列（CDS），包括PRKACAa、PRKACAb、PRKACBa、PRKACBb、PRKAR 1A、PRKAR 1B、PRKAR 2Aa、PRKAR 2Ab和PRKAR 2B，以及可能发生鱼类特异性基因组复制的PRKACA、PRKACB和PRKAR 2A。序列分析表明，草鱼PKA基因家族成员的蛋白质结构存在差异。草鱼PRKACAa、PRKACAb、PRKACBa和PRKACBb含有丝氨酸/苏氨酸蛋白激酶，而PRKAR 1A、PRKAR 1B、PRKAR 2Aa、PRKAR 2Ab和PRKAR 2B含有两个环核苷酸-单磷酸结合结构域。PRKACAa、PRKACBa、PRKACBb、PRKAR 1A、PRKAR 1B和PRKAR 2Aa含有10个编码外显子，PRKACAb和PRKAR 2Ab分别含有12个和5个编码外显子。在广泛的组织中检测到9种PKA亚型的信使RNA（mRNA），但其丰度显示组织依赖性表达模式。在衣霉素诱导的脂肪细胞脂解中，只有PRKACAb和PRKACBa的mRNA水平在脂肪细胞中显示出显著的增加（p
Protein kinase A (PKA), one of the most widely studied protein kinases, has many functions in cells, including regulating the metabolism of sugar and lipid. Here we identified nine isoforms of the PKA family in grass carp Ctenopharyngodon idella and obtained their complete coding sequences (CDS), including PRKACAa, PRKACAb, PRKACBa, PRKACBb, PRKAR1A, PRKAR1B, PRKAR2Aa, PRKAR2Ab and PRKAR2B, and PRKACA, PRKACB and PRKAR2A, which may experience fish-specific genome duplication. Sequence analysis showed that the predicted protein structures of PKA gene family members in grass carp were different. Grass carp PRKACAa, PRKACAb, PRKACBa, and PRKACBb contained serine/threonine protein kinases, while PRKAR1A, PRKAR1B, PRKAR2Aa, PRKAR2Ab and PRKAR2B contained two cyclic nucleotide-monophosphate binding domains. PRKACAa, PRKACBa, PRKACBb, PRKAR1A, PRKAR1B and PRKAR2Aa contained 10 coding exons, while PRKACAb and PRKAR2Ab consisted of 12 coding exons and 5 coding exons, respectively. The messenger RNA (mRNA) of the nine PKA isoforms was detected in a wide range of tissues, but their abundance showed tissue-dependent expression patterns. In tunicamycin-induced adipocyte lipolysis, only the mRNA levels of PRKACAb and PRKACBa showed a significant increase in adipocyte (p