Diagnostic utility of thyroid transcription factors Pax8 and TTF-2 (FoxE1) in thyroid epithelial neoplasms
Diagnostic utility of thyroid transcription factors Pax8 and TTF-2 (FoxE1) in thyroid epithelial neoplasms
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DOI：
10.1038/modpathol.3801002
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发表时间：
2008-02-01
期刊：
影响因子：
7.5
通讯作者：
Ghossein, Ronald
中科院分区：
文献类型：
--
作者：
Nonaka, Daisuke;Tang, Yunjia;Ghossein, Ronald
甲状腺特异性转录因子Pax 8、TTF-1和TTF-2对甲状腺器官发生和分化至关重要。与TTF-1相比，其他两种标记物在外科病理学中的研究较少。本研究的目的是评估这些标志物在甲状腺肿瘤的全谱分化，特别强调间变性癌的表达。共94例甲状腺肿瘤进行了研究：乳头状癌17例，滤泡性腺瘤18例，滤泡性癌16例，低分化癌7例，未分化癌28例，髓样癌8例。对这三种标志物进行免疫染色。Pax 8和TTF-2的抗体也应用于147例肺癌以及各种正常组织和恶性肿瘤。所有这三个标志物被认为是在乳头状癌，滤泡性腺瘤和癌，低分化癌中弥漫性的方式，而它们的表达在髓样癌是可变的。Pax 8在79%的未分化癌中表达，表达程度不同，而TTF-1和TTF-2分别仅在18%和7%的未分化癌中表达。TTF-2在所有其他肿瘤和非肿瘤组织（包括肺组织）中均为阴性。Pax 8在肾小管、输卵管、卵巢包涵体囊肿、淋巴滤泡以及肾癌、肾母细胞瘤、卵巢上皮瘤和卵巢癌中表达，而在正常组织和肺癌中不表达。Pax 8是一个有用的标记物，用于诊断间变性癌，特别是当鉴别诊断包括肺癌。在分化型甲状腺肿瘤中，三种转录因子的表达无显著差异。
Thyroid-specific transcription factors, Pax8, TTF-1, and TTF-2, are crucial for thyroid organogenesis and differentiation. Compared with TTF-1, the other two markers have scarcely been investigated in surgical pathology. The goal of this study is to evaluate the expressions of these markers in thyroid tumors of the full spectrum of differentiation, with special emphasis on anaplastic carcinomas. A total of 94 cases of thyroid neoplasms were studied: 17 papillary carcinomas, 18 follicular adenomas, 16 follicular carcinomas, 7 poorly differentiated carcinomas, 28 anaplastic carcinomas, and 8 medullary carcinomas. Immunostains for these three markers were performed. The antibodies to Pax8 and TTF-2 were also applied on 147 lung carcinomas as well as a variety of normal tissues and malignant tumors. All three markers were seen in papillary carcinomas, follicular adenomas and carcinomas, and poorly differentiated carcinomas in a diffuse manner, whereas their expressions in medullary carcinomas were variable. Pax8 was expressed in 79% of anaplastic carcinomas to a variable extent, whereas TTF-1 and TTF-2 were seen only in 18 and 7% of anaplastic carcinomas, respectively. TTF-2 was negative in all other neoplastic and non-neoplastic tissues including those of the lung. Pax8 was expressed in renal tubules, fallopian tubes, ovarian inclusion cysts, and lymphoid follicles as well as renal carcinoma, nephroblastoma, seminoma, and ovarian carcinoma, but not in normal tissue and carcinomas of the lung. Pax8 is a useful marker for the diagnosis of anaplastic carcinomas, particularly when the differential diagnosis includes pulmonary carcinoma. In differentiated thyroid neoplasms, no significant difference in expression was seen in all the three transcription factors.