The Oxygen Dilemma: A Severe Challenge for the Application of Monooxygenases?
The Oxygen Dilemma: A Severe Challenge for the Application of Monooxygenases?
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DOI：
10.1002/cbic.201600176
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发表时间：
2016-08-03
期刊：
影响因子：
--
通讯作者：
Hollmann F
中科院分区：
文献类型：
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作者：
Holtmann D;Hollmann F
单加氧酶是有前途的催化剂，因为它们原则上使有机化学家能够进行高选择性的氧官能化反应，而这是其他方法难以实现的。为此，单加氧酶需要还原当量，以允许在酶的活性位点还原激活分子氧。然而，这些还原当量通常直接或通过还原中间体（解偶联）传递给 O2，产生危险的活性氧并浪费宝贵的还原当量。氧困境源于单加氧酶对 O2 的依赖以及不希望的解偶联反应。通过这一贡献，我们希望引起人们对氧气困境的普遍认识，并讨论其本质和一些有希望的解决方案。
Monooxygenases are promising catalysts because they in principle enable the organic chemist to perform highly selective oxyfunctionalisation reactions that are otherwise difficult to achieve. For this, monooxygenases require reducing equivalents, to allow reductive activation of molecular oxygen at the enzymes' active sites. However, these reducing equivalents are often delivered to O2 either directly or via a reduced intermediate (uncoupling), yielding hazardous reactive oxygen species and wasting valuable reducing equivalents. The oxygen dilemma arises from monooxygenases' dependency on O2 and the undesired uncoupling reaction. With this contribution we hope to generate a general awareness of the oxygen dilemma and to discuss its nature and some promising solutions.