Marine mimivirus relatives are probably large algal viruses.
Marine mimivirus relatives are probably large algal viruses.
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DOI：
10.1186/1743-422x-5-12
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发表时间：
2008-01-23
期刊：
影响因子：
4.8
通讯作者：
Ogata H
中科院分区：
文献类型：
--
作者：
Monier A;Larsen JB;Sandaa RA;Bratbak G;Claverie JM;Ogata H
棘阿米巴拟菌病毒是已知最大的双链 DNA 病毒，其 1.2 Mb 基因组序列揭示了许多独特的特征。拟菌病毒在真核病毒中占有独立的谱系，其已知宿主仅包括棘阿米巴属物种。对马尾藻海宏基因组数据的分析首次表明拟菌病毒亲属的存在。现在，我们使用更大的海洋宏基因组数据集进一步证明了许多“拟病毒样”序列的存在。我们还表明，感染不同海洋藻类（Chrysochromulina ericina、Phaeocystis pouchetii、Pyramimonas orientalis）的三种藻病毒（CeV01、PpV01、PoV01）的 DNA 聚合酶序列与其在拟病毒中的同源物非常密切相关。我们的结果表明，在海洋环境中发现的大量拟菌病毒相关序列可能源自感染浮游植物的多种大型 DNA 病毒。因此，微藻构成了一个新的潜在宿主类别，可以在其中寻找拟病毒科的新物种。
Acanthamoeba polyphaga mimivirus is the largest known ds-DNA virus and its 1.2 Mb-genome sequence has revealed many unique features. Mimivirus occupies an independent lineage among eukaryotic viruses and its known hosts include only species from the Acanthamoeba genus. The existence of mimivirus relatives was first suggested by the analysis of the Sargasso Sea metagenomic data. We now further demonstrate the presence of numerous "mimivirus-like" sequences using a larger marine metagenomic data set. We also show that the DNA polymerase sequences from three algal viruses (CeV01, PpV01, PoV01) infecting different marine algal species (Chrysochromulina ericina, Phaeocystis pouchetii, Pyramimonas orientalis) are very closely related to their homolog in mimivirus. Our results suggest that the numerous mimivirus-related sequences identified in marine environments are likely to originate from diverse large DNA viruses infecting phytoplankton. Micro-algae thus constitute a new category of potential hosts in which to look for new species of Mimiviridae.