Abnormalities of male-specific FRU protein and serotonin expression in the CNS of fruitless mutants in Drosophila
Abnormalities of male-specific FRU protein and serotonin expression in the CNS of fruitless mutants in Drosophila
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DOI：
10.1523/jneurosci.21-02-00513.2001
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发表时间：
2001-01-15
影响因子：
5.3
通讯作者：
Hall, JC
中科院分区：
文献类型：
--
作者：
Lee, G;Hall, JC
果蝇中的无果基因产生雄性特有的蛋白质(FRU(M))，参与控制求偶。对表现出异常的男性求偶行为的fru突变体进行了Frum空间和时间模式的研究。该基因座的染色体断裂点消除了Frum。纯合的可存活突变体表现出一系列耐人寻味的缺陷。在FRU(1)男性中，缺少表达Frum的神经元簇或某些簇内的染色细胞，其他簇的信号强度降低，在新细胞中异位表达Frum。FRU(2)雄鼠在所有正常表达该基因的神经元中都表现出Frum表达的总体减少。Fru(4)和fru(Sat)突变体仅在极低水平的少量神经元中产生Frum信号，而在fru(3)雄性神经元中未检测到Frum信号。据推测，这一系列异常与这些突变体表现出的不同行为缺陷有关。这种异常包括男性之间的求爱，这被认为与大脑中5-羟色胺(5-HT)功能异常有关。然而，双标记未发现Frum和5-羟色胺在脑神经元中共表达。然而，在成年男性的腹神经节中发现了一组新发现的性二型Frum/5-HT阳性神经元。这些性二型神经元(S-ABG)投射到腹部与男性生殖有关的区域。在野生型雌鼠中，S-ABG神经元及其轴突的近端不染色或不着色，而在某些纯突变型雄鼠中表现出低于正常或无5-羟色胺的免疫反应，表明无果控制着这些细胞的形成或5-羟色胺的产生。
The fruitless gene in Drosophila produces male-specific protein (FRU (M)) involved in the control of courtship. FRUM spatial and temporal patterns were examined in fru mutants that exhibit aberrant male courtship. Chromosome breakpoints at the locus eliminated FRUM. Homozygous viable mutants exhibited an intriguing array of defects. In fru (1) males, there were absences of FRUM-expressing neuronal clusters or stained cells within certain clusters, reductions of signal intensities in others, and ectopic FRUM expression in novel cells. fru(2) males exhibited an overall decrement of FRUM expression in all neurons normally expressing the gene. fru(4) and fru(sat) mutants only produced FRUM in small numbers of neurons at extremely low levels, and no FRUM signals were detected in fru(3) males. This array of abnormalities was inferred to correlate with the varying behavioral defects exhibited by these mutants. Such abnormalities include courtship among males, which has been hypothesized to involve anomalies of serotonin (5-HT) function in the brain. However, double-labeling uncovered no coexpression of FRUM and 5-HT in brain neurons. Yet, a newly identified set of sexually dimorphic FRUM/5-HT-positive neurons was identified in the abdominal ganglion of adult males. These sexually dimorphic neurons (s-Abg) project toward regions of the abdomen involved in male reproduction. The s-Abg neurons and the proximal extents of their axons were unstained or absent in wild-type females and exhibited subnormal or no 5-HT immunoreactivity in certain fru-mutant males, indicating that fruitless controls the formation of these cells or 5-HT production in them.