Phase separation of Dri1 contributes to heterochromatin formation in Schizosaccharomyces pombe.
Phase separation of Dri1 contributes to heterochromatin formation in Schizosaccharomyces pombe.
复制标题
DOI：
10.17912/micropub.biology.000559
复制
发表时间：
2022
影响因子：
--
通讯作者：
Li, Fei
中科院分区：
文献类型：
--
作者：
Ban, Hyoju;Sun, Wenqi;Chen, Yong;Li, Fei
RNA结合蛋白Dri1通过RNAi途径和组蛋白去乙酰化酶（HDAC）促进异染色质组装。Dri1在其c端含有一个内在无序区（IDR）和三个锌指，这对其在异染色质沉默中起重要作用。IDR和锌指都参与了液-液相分离（LLPS）。在这项研究中，我们研究了Dri1的相分离性能。我们观察到Dri1在体外发生相分离。Dri1在体内也表现出类似液体的行为。结合我们之前的发现，我们的数据支持一个模型，即Dri1形成的相分离凝聚物可能有助于招募RNAi成分和HDAC来介导异染色质组装。
The RNA binding protein Dri1 facilitates heterochromatin assembly via the RNAi pathway and histone deacetylases (HDAC). Dri1 contains an intrinsically disordered region (IDR) and three zinc fingers at its C-terminus, which are important for its role in heterochromatin silencing. Both IDR and zinc fingers have been implicated in mediating liquid-liquid phase separation (LLPS). In this study, we investigated the phase separation properties of Dri1. We observed that Dri1 undergoes phase separation in vitro . Dri1 also exhibits liquid-like behavior in vivo . Combined with our previous findings, our data support a model in which the phase-separated condensates formed by Dri1 may help recruit RNAi components and HDAC to mediate heterochromatin assembly.