Digital microfluidics: a future technology in the newborn screening laboratory?
Digital microfluidics: a future technology in the newborn screening laboratory?
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DOI：
10.1053/j.semperi.2009.12.008
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发表时间：
2010-04
影响因子：
3.4
通讯作者：
Pamula V
中科院分区：
文献类型：
--
作者：
Millington DS;Sista R;Eckhardt A;Rouse J;Bali D;Goldberg R;Cotten M;Buckley R;Pamula V
使用串联质谱法扩大新生儿遗传性代谢性疾病筛查引起了人们对筛查其他可治疗疾病（包括溶酶体贮积病）的兴趣。扩张的限制包括劳动力和设备成本。我们描述了一种经济高效的新平台，可以减少结果报告的时间，并且可以执行需要不同平台的多重检测。目前用于新生儿筛查的免疫测定和酶活性测定已被转化为一次性微芯片，该微芯片被编程为分配、运输、混合、洗涤和孵育样本中的单个微滴，包括干血斑提取物和试剂，所有这些都在软件控制下。消耗的样本和试剂大约是同等台式检测所需的 1%。除了免疫学和酶测定之外，还使用相同的微芯片和控制设备演示了 DNA 扩增、扩增子检测和测序。最近，4 种不同酶活性的多重化也被证明具有可忽略的交叉污染。我们回顾与新生儿筛查相关的检测。
Expansion of newborn screening for inherited metabolic disorders using tandem mass spectrometry has generated interest in screening for other treatable conditions, including lysosomal storage diseases. Limitations to expansion include labor and equipment costs. We describe a cost-effective new platform that reduces the time to result reporting and can perform multiplexing assays requiring different platforms. Immunoassays and enzyme activity assays currently used in newborn screening have been translated to a disposable microchip programmed to dispense, transport, mix, wash, and incubate individual microdroplets from specimens, including dried blood spot extracts, and reagents all under software control. The specimen and reagents consumed are approximately 1% of those required by equivalent bench assays. In addition to immunologic and enzymatic assays, DNA amplification, amplicon detection, and sequencing have been demonstrated using the same microchips and control equipment. Recently, the multiplexing of 4 different enzyme activities has also been demonstrated with negligible cross-contamination. We review assays relevant to newborn screening.
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影响因子：
5.1
作者：
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通讯作者：
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影响因子：
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影响因子：
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影响因子：
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作者：
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通讯作者：
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