Structural analysis of sulfated fucan from Saccharina japonica by electrospray ionization tandem mass spectrometry
Structural analysis of sulfated fucan from Saccharina japonica by electrospray ionization tandem mass spectrometry
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DOI：
10.1016/j.carres.2012.12.004
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发表时间：
2013-03-22
影响因子：
3.1
通讯作者：
Zhang, Quanbin
中科院分区：
文献类型：
--
作者：
Jin, Weihua;Guo, Zhimou;Zhang, Quanbin
通过用DMSO-MeOH处理来自日本竹荪的岩藻依聚糖硫酸酯，导致聚合物分子通过甲醇分解部分降解，产生中性、单硫酸化和二硫酸化岩藻寡糖的混合物，其形式为甲基糖苷。通过ESI-MS和ESI-CID-MS/MS表征这些寡聚片段。发现寡糖结构与主要由在位置4和2处硫酸化的（1 -> 3）-连接的岩藻糖残基构成的多糖主链一致。(C)2012爱思唯尔有限公司保留所有权利。
Desulfation of a fucoidan from Saccharina japonica by treatment with DMSO-MeOH resulted in partial degradation of polymeric molecules by methanolysis giving rise to a mixture of neutral, monosulfated, and disulfated fucooligosaccharides in the form of methyl glycosides. These oligomeric fragments were characterized by ESI-MS and ESI-CID-MS/MS. It was found that oligosaccharide structures coincided with the polysaccharide backbone built up mainly of (1 -> 3)-linked fucose residues sulfated at positions 4 and 2. (C) 2012 Elsevier Ltd. All rights reserved.