Plant disease resistance genes encode members of an ancient and diverse protein family within the nucleotide-binding superfamily
Plant disease resistance genes encode members of an ancient and diverse protein family within the nucleotide-binding superfamily
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DOI：
10.1046/j.1365-313x.1999.t01-1-00606.x
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发表时间：
1999-11-01
期刊：
影响因子：
7.2
通讯作者：
Young, ND
中科院分区：
文献类型：
--
作者：
Meyers, BC;Dickerman, AW;Young, ND
核苷酸结合位点（NBS）是许多植物抗病基因产物的特征结构域。越来越多的NBS编码序列通过基因克隆、简并引物PCR扩增和基因组测序项目被鉴定。对14个已知的植物抗病基因和400多个同源基因的NBS结构域进行了分析，这些基因来自单子叶植物、双子叶植物和针叶植物的26个属。两组不同的序列被确定，表明在进化过程中的分歧和这些序列的古老起源。一组由编码具有Toll/白细胞介素-1受体同源性（TIR）的N-末端结构域的序列组成，包括已知的抗性基因N、M、L 6、RPP 1和RPP 5。令人惊讶的是，在随机基因组序列和大量EST的搜索中，单子叶植物中完全没有这一组。第二组包含单子叶植物和双子叶植物序列，包括已知的抗性基因RPS 2、RPM 1、I2、Mi、Dm 3、Pi-B、Xa 1、RPP 8、RPS 5和Prf。包含NBS结构域的保守基序中的氨基酸特征清楚地区分了这两组。拟南芥基因组估计含有约200个编码相关NBS基序的基因; TIR序列更丰富，数量超过非TIR序列的三倍。目前数据库中的拟南芥NBS序列位于大约21个基因组簇和14个分离的基因座中。NBS编码序列可能在水稻中更普遍。这些序列在植物界中的广泛分布以及它们在拟南芥和水稻基因组中的普遍存在表明它们在植物中是古老的、多样的和常见的。序列推断表明，这些基因编码一类新的核苷酸结合蛋白。
The nucleotide binding site (NBS) is a characteristic domain of many plant resistance gene products. An increasing number of NBS-encoding sequences are being identified through gene cloning, PCR amplification with degenerate primers, and genome sequencing projects. The NBS domain was analyzed from 14 known plant resistance genes and more than 400 homologs, representing 26 genera of monocotyledonous, dicotyle-donous and one coniferous species. Two distinct groups of diverse sequences were identified, indicating divergence during evolution and an ancient origin for these sequences. One group was comprised of sequences encoding an N-terminal domain with Toll/Interleukin-1 receptor homology (TIR), including the known resistance genes, N, M, L6, RPP1 and RPP5. Surprisingly, this group was entirely absent from monocot species in searches of both random genomic sequences and large collections of ESTs. A second group contained monocot and dicot sequences, including the known resistance genes, RPS2, RPM1, I2, Mi, Dm3, Pi-B, Xa1, RPP8, RPS5 and Prf. Amino acid signatures in the conserved motifs comprising the NBS domain clearly distinguished these two groups. The Arabidopsis genome is estimated to contain approximately 200 genes that encode related NBS motifs; TIR sequences were more abundant and outnumber non-TIR sequences threefold. The Arabidopsis NBS sequences currently in the databases are located in approximately 21 genomic clusters and 14 isolated loci. NBS-encoding sequences may be more prevalent in rice. The wide distribution of these sequences in the plant kingdom and their prevalence in the Arabidopsis and rice genomes indicate that they are ancient, diverse and common in plants. Sequence inferences suggest that these genes encode a novel class of nucleotide-binding proteins.