Genetic similarity among cultivars of Phyllostachys pubescens
Genetic similarity among cultivars of Phyllostachys pubescens
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DOI：
10.1007/s00606-008-0104-1
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发表时间：
2009-01-01
影响因子：
1.9
通讯作者：
Fang, Wei
中科院分区：
文献类型：
--
作者：
Lin, Xin Chun;Ruan, Xiao Sai;Fang, Wei
毛竹是我国最重要的经济竹种，广泛分布于我国南方。本种有10多个栽培品种，但其亲缘关系尚不清楚。利用AFLP和ISSR技术对10个毛白杨品种和2个近缘种进行了遗传相似性分析。15对AFLP引物共扩增出827条带，其中多态性条带495条; 16对ISSR引物共扩增出231条带，其中多态性条带154条。统计分析表明，两组分子标记的遗传相似性矩阵具有显著相关性（R = 0.959，P = 0.013）。利用AFLP和ISSR分子标记构建的聚类图可将10个相似性较高的毛竹品种清晰地划分为3个类群，遗传距离在0.023 ~ 0.108之间。我们的研究结果表明，这些分子标记是有用的遗传分类品种或品种的一个物种，特别是竹种。
Phyllostachys pubescens is the most important economic bamboo species in China, which grows widely in the South of China. There are more than ten cultivars in this species but their genetic relationship still remains unknown. We used both amplified fragment length polymorphism (AFLP) and inter-simple sequence repeat (ISSR) techniques to determine genetic similarity among ten cultivars of P. pubescens and two related species. Eight hundred and twenty seven bands, in which 495 are polymorphic, were detected using 15 pairs of AFLP primers whereas total 231 bands, in which 154 bands are polymorphic, were scored using 16 ISSR primers. Statistic analysis showed that the genetic similarity matrices obtained from these two sets of molecular markers had a significant correlation (R = 0.959, P = 0.013). The dendrogram generated with AFLP and ISSR markers could clearly genetically identify ten cultivars of P. pubescens that had high similarity with genetic distances ranging from 0.023 to 0.108, and could be divided into three groups based on their genetic variation and similarity. Our results suggest that these molecular markers are useful to genetically classify cultivars or varieties of a species, particularly a bamboo species.