Pathogenicity of duck plague and innate immune responses of the Cherry Valley ducks to duck plague virus.
Pathogenicity of duck plague and innate immune responses of the Cherry Valley ducks to duck plague virus.
复制标题
鸭瘟的致病性及樱桃谷鸭对鸭瘟病毒的先天免疫反应。
DOI：
10.1038/srep32183
复制
发表时间：
2016-08-24
影响因子：
4.6
通讯作者：
Wei L
中科院分区：
文献类型：
--
作者：
Li N;Hong T;Li R;Guo M;Wang Y;Zhang J;Liu J;Cai Y;Liu S;Chai T;Wei L
鸭瘟是由鸭瘟病毒(DPV)引起的一种急性传染病。为了更好地了解鸭瘟病毒对雏鸭的致病机理，进行了雏鸭感染试验。结果表明，感染雏鸭出现典型症状。DPV可以在许多组织中快速复制，导致病理损害，特别是在脾上。实时定量聚合酶链式反应显示，许多先天免疫相关基因在脑内表达上调，抗病毒先天免疫反应已建立，但不足以限制病毒复制。相反，尽管许多主要的模式识别受体(PRRs)在脾中的表达增加，但大多数细胞因子的表达却下降。我们的研究表明，DPV是一种泛嗜性病毒，可以在组织中快速复制，引起严重的病理损害，但在脾和脑中的免疫反应不同。据我们所知，这是第一次系统地探索DPV感染鸭的免疫基因的表达谱。为进一步研究鸭瘟的致病性奠定了基础。
Duck plague caused by duck plague virus (DPV) is an acute and contagious disease. To better understand the pathogenic mechanism of duck plague virus in ducklings, an infection experiment was performed. Our results showed that typical symptoms were observed in the infected ducklings. DPV could replicate quickly in many tissues, leading to pathological lesions, especially on the spleen. Real-time quantitative PCR demonstrated that expression of many innate immune-related genes was mostly up-regulated in the brain and the antiviral innate immune response was established, but not sufficient to restrict viral replication. In contrast, although the expression of many major pattern recognition receptors (PRRs) increased in the spleen, the expression of most cytokines was declined. Our study indicates that DPV is a pantropic virus that can replicate rapidly in tissues, causing serious pathological lesions but the immune responses are different in the spleen and brain. To our knowledge, this is the first report to systematically explore the expression profiles of the immune genes in the DPV-infected ducks. Our data provide a foundation for further study of the pathogenicity of duck plague.