African 2, a Clonal Complex of Mycobacterium bovis Epidemiologically Important in East Africa
African 2, a Clonal Complex of Mycobacterium bovis Epidemiologically Important in East Africa
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DOI：
10.1128/jb.00750-10
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发表时间：
2011-02-01
影响因子：
3.2
通讯作者：
Smith, Noel H.
中科院分区：
文献类型：
--
作者：
Berg, Stefan;Garcia-Pelayo, M. Carmen;Smith, Noel H.
我们已经从乌干达、布隆迪、坦桑尼亚和埃塞俄比亚的牛身上鉴定出一个高频率分离的牛分枝杆菌克隆性复合体。我们将这组相关的牛分枝杆菌命名为牛分枝杆菌非洲2(AF2)克隆复合体。AF2菌株是由一个特定的染色体缺失(RDAf2)定义的，并可以通过在其孢子型模式中缺少间隔区3至7来鉴定。对来自阿尔及利亚、马里、乍得、尼日利亚、喀麦隆、南非和莫桑比克的牛分枝杆菌分离株的缺失分析没有发现AF2克隆复合体的任何菌株，这表明牛分枝杆菌的克隆复合体定位于东非。在非洲以外的分离株中，AF2克隆复合体的特殊配子型模式很少被鉴定，并且少数被发现和测试的分离株在RDAf2基因座上是完整的。我们的结论是AF2克隆复合体定位于东非的牛。我们发现，牛分枝杆菌AF2克隆复合体的菌株一般具有四个或更多拷贝的插入序列IS6110，而从牛分离的大多数牛分枝杆菌菌株被认为只携带一个或几个拷贝。
We have identified a clonal complex of Mycobacterium bovis isolated at high frequency from cattle in Uganda, Burundi, Tanzania, and Ethiopia. We have named this related group of M. bovis strains the African 2 (Af2) clonal complex of M. bovis. Af2 strains are defined by a specific chromosomal deletion (RDAf2) and can be identified by the absence of spacers 3 to 7 in their spoligotype patterns. Deletion analysis of M. bovis isolates from Algeria, Mali, Chad, Nigeria, Cameroon, South Africa, and Mozambique did not identify any strains of the Af2 clonal complex, suggesting that this clonal complex of M. bovis is localized in East Africa. The specific spoligotype pattern of the Af2 clonal complex was rarely identified among isolates from outside Africa, and the few isolates that were found and tested were intact at the RDAf2 locus. We conclude that the Af2 clonal complex is localized to cattle in East Africa. We found that strains of the Af2 clonal complex of M. bovis have, in general, four or more copies of the insertion sequence IS6110, in contrast to the majority of M. bovis strains isolated from cattle, which are thought to carry only one or a few copies.