STUDIES ON THE LINKAGE OF ENERGY-METABOLISM AND NEURONAL-ACTIVITY IN THE ISOLATED PERFUSED RAT-BRAIN
STUDIES ON THE LINKAGE OF ENERGY-METABOLISM AND NEURONAL-ACTIVITY IN THE ISOLATED PERFUSED RAT-BRAIN
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DOI：
10.1111/j.1471-4159.1980.tb06266.x
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发表时间：
1980-01-01
影响因子：
4.7
通讯作者：
WICKOP, G
中科院分区：
文献类型：
--
作者：
DIRKS, B;HANKE, J;WICKOP, G
分离的大鼠脑制剂用无葡萄糖(血糖)的培养液灌流。测定脑组织中高能磷酸[肌酸磷酸、ATP、ADP、AMP]、糖酵解途径底物[糖原、葡萄糖、葡萄糖-6-磷酸、果糖-6-磷酸、乳酸、丙酮酸]、游离氨基酸[谷氨酸、谷氨酰胺、天门冬氨酸]、乙酰胆碱以及己糖激酶活性在细胞内的分布。对脑电进行目测评估。灌流后皮质组织中糖酵解底物、谷氨酸和谷氨酰胺水平降低，天冬氨酸水平升高，GABA水平不变。葡萄糖灌流15min左右，高能磷酸盐含量无明显变化，20min后明显下降。离体脑的脑电在开始无糖灌流后迅速变化，12~15min后变为等电。高血糖灌流(35 mmol葡萄糖/L灌注液)不改变离体脑的能量代谢。在灌流介质中加入硫喷妥钠后，脑组织能量代谢和脑电活动的破坏延缓。皮质组织中测得的可溶性己糖激酶活性在糖化灌流后降低，而在硫喷妥钠后增强。高血糖灌流不影响己糖激酶在细胞内的分布。血糖升高可使离体脑纹状体内乙酰胆碱含量显著降低，硫喷妥钠可使下丘脑乙酰胆碱含量显著升高。结合在线粒体膜上的己糖激酶可能在能量代谢与神经元活动的关系中发挥重要作用。
An isolated rat brain preparation was perfused using glucose-free (aglycemic) media. The high-energy phosphates [creatine phosphate, ATP, ADP, AMP], substrates of the glycolytic pathway [glycogen, glucose, glucose-6-phosphate, fructose-6-phosphate, lactate, pyruvate], free amino acids [glutamate, glutamine, aspartate], acetylcholine and the intracellular distribution of hexokinase activity were determined in brain tissues. The EEG was evaluated visually. The levels of glycolytic substrates, glutamate and glutamine in cortical tissue decreased after aglycemic perfusion; the aspartate level increased and the GABA level remained unchanged. The high-energy phosphate content seemed unaffected for about 15 min of aglycemic perfusion and fell significantly after 20 min. The EEG of the isolated brain changed rapidly after starting aglycemic perfusion and became isoelectric after 12-15 min. Hyperglycemic perfusion (35 mmol glucose/l perfusion medium) did not alter the energy metabolism of the isolated brain. The breakdown of cerebral energy metabolism and EEG activity was postponed when thiopental was added to the perfusion medium. The soluble hexokinase activity measured in cortical tissue was reduced after aglycemic perfusion and was enhanced after thiopental. Hyperglycemic perfusion did not influence the intracellular hexokinase distribution. The acetylcholine level in the striatum of the isolated rat brain was significantly decreased by aglycemia and was increased in hypothalamus by thiopental. Hexokinase bound to the mitochondrial membrane may play an important role in the relationship of energy metabolism and neuronal activity.