Noticeable Decreased Expression of Tenascin-X in Calcific Aortic Valves
Noticeable Decreased Expression of Tenascin-X in Calcific Aortic Valves
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DOI：
10.3109/03008207.2012.702818
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发表时间：
2012-01-01
影响因子：
2.9
通讯作者：
Oda, Teiji
中科院分区：
文献类型：
--
作者：
Matsumoto, Ken-Ichi;Satoh, Kazumi;Oda, Teiji
主动脉瓣钙化导致主动脉瓣狭窄，并正在成为老年人群中常见的瓣膜疾病。了解这种瓣膜病变的分子机制对于揭示与这种疾病的发展和进展相关的潜在生物标志物非常重要。为了鉴定钙化主动脉瓣（CAV）与邻近正常瓣膜组织中差异表达的蛋白质，采用定量蛋白质组学方法，以同量异序标记进行绝对和相对定量标记，然后采用纳米液相色谱基质辅助激光解吸/电离飞行时间串联质谱法对组织中差异表达的蛋白质进行综合分析。蛋白质组学分析显示，105个蛋白质差异表达的CAV与相邻的正常瓣膜组织相比，具有较高的置信度。在34种蛋白质中发现了显著增加的表达（>= 1.3倍），而在24种蛋白质中发现了减少的表达（
Calcification of aortic valves results in valvular aortic stenosis and is becoming a common valvular condition in elderly populations. An understanding of the molecular mechanisms of this valve lesion is important for revealing potential biomarkers associated with the development and progression of this disease. In order to identify proteins that are differentially expressed in calcific aortic valves (CAVs) compared with those in adjacent normal valvular tissues, comprehensive analysis of differentially expressed proteins in the tissues was done by a quantitative proteomic approach with isobaric tag for absolute and relative quantitation labeling followed by nanoliquid chromatography matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry. The proteomic analysis revealed 105 proteins differentially expressed in CAVs in contrast to adjacent normal valvular tissues with high confidence. Significantly increased expression (>= 1.3-fold) was found in 34 proteins, whereas decreased expression (