Three-dimensional imaging of biological complexity
Three-dimensional imaging of biological complexity
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DOI：
10.1016/s1047-8477(02)00019-9
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发表时间：
2002-04-01
影响因子：
3
通讯作者：
Mannella, CA
中科院分区：
文献类型：
--
作者：
Frank, J;Wagenknecht, T;Mannella, CA
在过去的5年中，由于仪器和计算速度的进步，使用电子显微镜的三维成像技术在两个领域得到了很大的改进：细胞器或细胞切片的电子断层扫描和从单个粒子重建大分子。李氏埋藏法使标本在接近自然的条件下保存的程度有了突破。目前的挑战是将电子断层成像的分辨率提高到可以在细胞背景下识别大分子特征的程度。我们展示了两个应用领域的例子：肌肉三联体连接的结构和线粒体的结构和精细结构。随着冷冻切片技术的完善，我们希望能够将断层摄影术应用于高压冷冻组织切片。(C)2002 Elsevier Science（美国）。All rights reserved.
Over the past 5 years, thanks to advances in both instrumentation and computational speed, three-dimensional imaging techniques using the electron microscope have been greatly improved in two areas: electron tomography of cell organelles or cell sections and reconstruction of macromolecules from single particles. lee embedment has brought a breakthrough in the degree of preservation of specimens under close-to-native conditions. The current challenge is to push the resolution of electron tomographic imaging to a point where macromolecular signatures can be recognized within the cellular context. We show first progress toward this goal by examples in two areas of application: the structure of the muscle triad junction and the architecture and fine structure of mitochondria. As techniques of cryo-microtomy are perfected, we hope to be able to apply tomography to high-pressure frozen sections of tissue. (C) 2002 Elsevier Science (USA). All rights reserved.