Determination of lesion volume by MRI and stereology in a macaque model of tuberculosis
Determination of lesion volume by MRI and stereology in a macaque model of tuberculosis
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DOI：
10.1016/j.tube.2009.09.002
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发表时间：
2009-11-01
期刊：
影响因子：
3.2
通讯作者：
Dennis, M. J.
中科院分区：
文献类型：
--
作者：
Sharpe, S. A.;Eschelbach, E.;Dennis, M. J.
需要灵敏和可重复的方法来测量结核分枝杆菌实验性攻击后对宿主的影响，以确定新疫苗赋予的保护程度。在这里，我们比较了不同的临床和死后疾病负担的措施预测宿主对增加剂量的M的反应。恒河猴和食蟹猴的结核病。从M.结核病感染的动物被福尔马林固定并离体扫描。与胸部X线摄影、病理学评分、体重和体温变化以及红细胞血红蛋白浓度和沉降率相比，总肺病变体积相对于固定的全肺体积在指示疾病负荷方面具有上级优势。总病变体积准确反映了M激发剂量的差异。通过气溶胶递送的范围为30至500 CFU的结核病。从MR图像中确定的总病变体积表明恒河猴和食蟹猴对M的敏感性存在种属依赖性差异。肺结核感染。MR体视学提供了一种准确、可量化且相对简单的评估，可以在实验室之间轻松标准化，并应成为疾病进展或疫苗有效性临床评估的重要组成部分。(C)2009爱思唯尔有限公司保留所有权利。
Sensitive and reproducible methods are needed to measure the impact on the host following experimental challenge with Mycobacterium tuberculosis, in order to determine the degree of protection conferred by new vaccines. Here we compare how well different clinical and post-mortem measures of disease burden predict the response by the host to increasing doses of M. tuberculosis in rhesus and cynomolgus macaques. The total lung and lesion volume was quantified from magnetic resonance imaging (MRI) digital stacks obtained from lungs of M. tuberculosis infected animals that were formalin fixed and scanned ex-vivo. The total lung lesion volume relative to the fixed whole lung volume was superior at indicating disease burden when compared to thoracic radiography, pathology scores, changes in body weight and temperature, as well as erythrocyte haemoglobin concentrations and sedimentation rate. The total lesion volume accurately reflected differences in challenge doses of M. tuberculosis that ranged from 30 to 500 CFU delivered by aerosol. The determination of total lesion volume from MR images demonstrated a species-dependent difference between rhesus and cynomolgus macaques in susceptibility to M. tuberculosis infection. MR stereology provides an accurate, quantifiable and relatively simple assessment, which can be easily standardized between laboratories and should form an essential component of the clinical assessment of disease progression, or vaccine efficacy. (C) 2009 Elsevier Ltd. All rights reserved.