Application of GC/MS-Based Metabonomic Profiling in Studying the Therapeutic Effects of Aconitum carmichaeli with Ampelopsis japonica Extract on Collagen-Induced Arthritis in Rats
Application of GC/MS-Based Metabonomic Profiling in Studying the Therapeutic Effects of Aconitum carmichaeli with Ampelopsis japonica Extract on Collagen-Induced Arthritis in Rats
复制标题
基于GC/MS的代谢组学分析在研究乌头与蛇葡萄提取物对大鼠胶原性关节炎的治疗作用中的应用
DOI：
10.3390/molecules24101934
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发表时间：
2019-05-02
期刊：
影响因子：
4.6
通讯作者：
Song, Lili
中科院分区：
文献类型：
--
作者：
Jin, Hua;Ma, Ningning;Song, Lili
乌头加葡萄（AA）是一种经典的中药配方。有很多例子表明AA可用于治疗类风湿性关节炎，但其作用机制尚不完全清楚。本研究选择胶原诱导关节炎（CIA）作为类风湿关节炎（RA）模型。各治疗组大鼠自关节炎发病第7天起（至第42天）连续给予乌头（AC）、日本蛇尾（AJ）和乌头+日本蛇尾（AA），每天1次口服。结果表明，AA不仅能显著减轻大鼠足跖肿胀，还能提高血清中TNF-α和IL-6的水平。建立了基于gc - ms的尿液代谢组学，分析了RA大鼠的代谢谱，并采用偏最小二乘判别分析（PLS-DA）和支持向量机（SVM）方法鉴定了21种生物标志物。支持向量机方法对21个生物标志物的预测率为100%。21项生物标志物中的20项，包括d -半乳糖、肌醇和甘油，在给予AA后逐渐恢复到正常水平。代谢组学途径分析（MetPA）发现了半乳糖代谢、甘油脂代谢和肌醇磷酸代谢三个相关的代谢途径，解释了AA治疗类风湿性关节炎的机制。本研究为了解复方中药对类风湿关节炎的治疗效果及可能的治疗机制提供了新的思路。
Aconitum carmichaeli with Ampelopsis japonica (AA) is a classical traditional Chinese medicine (TCM) formula. There are a lot of examples showing that AA can be used to treat rheumatoid arthritis, but its mechanism of action is still not completely clear. In this research, collagen-induced arthritis (CIA) was chosen as a rheumatoid arthritis (RA) model. Rats of treated groups were continuously administered Aconitum carmichaeli (AC), Ampelopsis japonica (AJ) and Aconitum carmichaeli + Ampelopsis japonica (AA) orally once a day from the day after the onset of arthritis (day 7) until day 42. The results showed that AA not only significantly reduced paw swelling, but also improved the levels of TNF-α and IL-6 in serum. GC-MS-based urine metabonomics was established to analysis metabolic profiles and 21 biomarkers of RA rats were identified by the Partial Least Squares Discriminant Analysis (PLS-DA) and Support Vector Machine (SVM) methods. The prediction rate of the SVM method for the 21 biomarkers was 100%. Twenty of 21 biomarkers, including D-galactose, inositol and glycerol, gradually returned to normal levels after administration of AA. Metabolomic Pathway Analysis (MetPA) generated three related metabolic pathways—galactose metabolism, glycerolipid metabolism and inositol phosphate metabolism—which explain the mechanism of AA treatment of rheumatoid arthritis. This research provides a better understanding of the therapeutic effects and possible therapeutic mechanism of action of a complex TCM (AA) on rheumatoid arthritis.