Cell lineage-specific transcriptome analysis for interpreting cell fate specification of proembryos.
Cell lineage-specific transcriptome analysis for interpreting cell fate specification of proembryos.
复制标题
用于解释原胚细胞命运规范的细胞谱系特异性转录组分析。
DOI：
10.1038/s41467-020-15189-w
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发表时间：
2020
影响因子：
16.6
通讯作者：
Sun Meng-Xiang
中科院分区：
文献类型：
--
作者：
Zhou Xuemei;Liu Zhenzhen;Shen Kun;Zhao Peng;Sun Meng-Xiang
在拟南芥中，受精卵经历不对称的细胞分裂，建立了早期原胚的前两种不同的细胞类型，顶端细胞和基底细胞。然而，指导顶端和基底细胞发育分化的全基因组转录活性仍然未知。在这里，我们提出了一个全面的顶端和基底细胞谱系的转录组分析，揭示不同的分子途径在细胞谱系规范。选择性删除遗传转录本和特异性从头转录有助于建立细胞系特异性细胞命运指定途径。从1-细胞胚胎阶段开始，胚胎相关通路在顶端细胞系中被特异性激活，但在基底细胞系中发现了向胚柄特异性通路的快速转录组重构。此外，长非编码RNA和选择性剪接亚型可能参与细胞谱系特化。这项工作也提供了一个有价值的谱系特异性转录组资源，以阐明顶端和基底细胞谱系在全基因组范围内的分歧的分子途径。
InArabidopsis, a zygote undergoes asymmetrical cell division that establishes the first two distinct cell types of early proembryos, apical and basal cells. However, the genome-wide transcriptional activities that guide divergence of apical and basal cell development remain unknown. Here, we present a comprehensive transcriptome analysis of apical and basal cell lineages, uncovering distinct molecular pathways during cell lineage specification. Selective deletion of inherited transcripts and specific de novo transcription contribute to the establishment of cell lineage-specific pathways for cell fate specification. Embryo-related pathways have been specifically activated in apical cell lineage since 1-cell embryo stage, but quick transcriptome remodeling toward suspensor-specific pathways are found in basal cell lineage. Furthermore, long noncoding RNAs and alternative splicing isoforms may be involved in cell lineage specification. This work also provides a valuable lineage-specific transcriptome resource to elucidate the molecular pathways for divergence of apical and basal cell lineages at genome-wide scale.