Diurnal changes of colonic motility and regulatory factors for colonic motility in Suncus murinus
Diurnal changes of colonic motility and regulatory factors for colonic motility in Suncus murinus
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鼩鼠结肠运动的日变化及结肠运动的调控因素
DOI：
10.1111/nmo.14302
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发表时间：
2021
影响因子：
3.5
通讯作者：
Sakata Ichiro
中科院分区：
文献类型：
--
作者：
Kobayashi Yuki;Takemi Shota;Sakai Takafumi;Shibata Chikashi;Sakata Ichiro
BackgroundThe aim of this study was investigate the fundamental muscus murinus in the house muscus（Suncus murinus）as a established animal model of gut motility.MethodsTo measure gut motility in free-moving conscious suncus，strain gauge force transducers were implanted on serosa of colon and gastric body.Key ResultsWe recorded daily changes in colonic motility and observed the relationship between feeding and colonic motility.巨大的迁移性收缩（GMCs）的结肠总是在排便期间检测到，并往往在黑暗期间增加，从而表明结肠运动具有昼夜节律。鉴于在黑暗时期喂食后立即观察到在suncus中的GMC，我们假设在suncus中发生胃结肠反射，与在人类和狗中观察到的相似。我们还研究了调节suncus GMCs的因素。5-HT静脉给药（100微克/千克），P物质（10和100 µg/kg），降钙素基因相关肽（10 µg/kg）和α2肾上腺素能受体拮抗剂育亨宾（0.5、1和3 mg/kg）诱导GMC样收缩，胃内和结肠内给予瞬时受体电位香草素1激动剂，结论与推论这些结果表明，在suncus结肠运动的基本机制是类似的，在人类和狗，因此，我们建议suncus可以作为一种新的小动物模型，研究结肠运动。
BackgroundThe aim of this study was to investigate the fundamental mechanisms of colonic motility in the house musk suncus (Suncus murinus) as an established animal model of gut motility.MethodsTo measure gut motility in free‐moving conscious suncus, strain gauge force transducers were implanted on the serosa of the colon and gastric body.Key ResultsWe recorded diurnal changes in colonic motility and observed the relationship between feeding and colonic motility. Giant migrating contractions (GMCs) of the colon were invariably detected during defecation and tended to increase during the dark period, thereby indicating that colonic motility has a circadian rhythm. Given that GMCs in the suncus were observed immediately after feeding during the dark period, we assume the occurrence of a gastrocolic reflex in suncus, similar to that observed in humans and dogs. We also examined the factors that regulate suncus GMCs. Intravenous administration of 5‐HT (100 µg/kg), substance P (10 and 100 µg/kg), calcitonin gene‐related peptide (10 µg/kg), and α2 adrenergic receptor antagonist yohimbine (0.5, 1, and 3 mg/kg) induced GMC‐like contractions, as did intragastric and intracolonic administration of the transient receptor potential vanilloid 1 agonist, capsaicin (1 mg/kg).Conclusions & InferencesThese results indicate that the fundamental mechanisms of colonic motility in suncus are similar to those in humans and dogs, and we thus propose that suncus could serve as a novel small animal model for studying colonic motility.