Immunological-chromatographic analysis.
Immunological-chromatographic analysis.
复制标题
免疫色谱分析。
DOI：
10.1016/s0021-9673(01)94003-8
复制
发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Regnier,FE
中科院分区：
文献类型：
--
作者：
Janis,LJ;Regnier,FE
采用串联免疫亲和色谱柱和常规高效液相色谱柱，配以一个切换阀，用于单一和多组分抗原样品的分析。将多抗或单抗疏水吸附或共价固定在聚苯乙烯-二乙烯基苯大孔填料上，制备免疫亲和柱。这些柱的容量在至少77个循环中保持恒定。在亚微克水平上分析大分子抗原。将结合在免疫亲和柱上的抗原解吸并集中在常规分析柱上。分析柱上的梯度洗脱将解吸的抗原(S)从干扰物种中分离出来，并允许分析与免疫亲和色谱柱结合的所有物种。免疫层析分析有助于纯化和鉴别具有相似三维结构的多肽，如几种溶菌酶变异体。
Tandem immunoaffinity and conventional high-performance liquid chromatography columns, coupled with a switching valve, were used for the analysis of single and multicomponent antigen samples. Immunoaffinity columns were prepared by hydrophobic adsorption or covalent immobilization of poly- or monoclonal anti-bodies on macroporous poly(styrene-divinylbenzene) packing materials. Capacities of these columns were constant through at least 77 cycles. Macromolecular antigens were analyzed at submicrogram levels. Antigens bound to be immunoaffinity column were desorbed and concentrated on a conventional analytical column. Gradient elution on the analytical column separated the desorbed antigen(s) from interfering species and permitted the analysis of all species which bound to the immunoaffinity column. Immunological-chromatographic analysis was useful for purification and discrimination of polypeptides of similar three dimensional structures, such as several lysozyme variants.