Effects of the lpr/lpr mutation on T and B cell populations in the lamina propria of the small intestine, a mucosal effector site.
Effects of the lpr/lpr mutation on T and B cell populations in the lamina propria of the small intestine, a mucosal effector site.
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lpr/lpr 突变对小肠固有层（粘膜效应位点）中 T 和 B 细胞群的影响。
DOI：
10.1093/intimm/4.9.959
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发表时间：
1992
影响因子：
4.4
通讯作者：
McGhee,JR
中科院分区：
文献类型：
--
作者：
Aicher,WK;Fujihashi,K;Yamamoto,M;Kiyono,H;Pitts,AM;McGhee,JR
MRL 小鼠患有淋巴增殖性疾病，其特征是 α/β T 细胞受体 + (TCR+) B220/6B2 + CD4−CD8−T 细胞 [淋巴细胞增殖 (Ipr) T 细胞] 数量增加，研究了 Ipr/lpr 突变对胃肠道 (GI) 粘膜免疫系统的影响。我们分析了 Ipr 基因突变对派尔氏淋巴结 (PP) 和固有层淋巴细胞 (LPL) 中 T 和 B 细胞群的影响，分别作为胃肠道中主要 IgA 诱导组织和效应组织的例子。正常小鼠 PP 含有致力于 IgA（表面 IgA+）的 B 细胞，但只有少量 B 细胞产生 IgA。然而，MRL 小鼠的 PP 中自发 IgA 和 IgG 合成增强。此外，我们现在已经证明 MRL 小鼠的 PP 中充满了 Ipr T 细胞。有趣的是，即使在老年动物中，MRL 小鼠的 LPL 中 Ipr T 细胞的数量并不多。有趣的是，与对照小鼠相比，MRL 中固有层中 CD4+ 与 CD8+T 细胞的比例较低，并且 CD8+T 细胞亚群实际上在自身免疫小鼠的 LPL 中占主导地位。此外，MRL Ipr/lpr小鼠LPL中γ/δ TCR+T细胞的数量显着增加，特别是在6周龄和12周龄的MRL Ipr/lpr小鼠中。当分析LPL中B细胞的同种型分布时，与MRL+/+或正常对照小鼠相比，MRL Ipr/lpr小鼠没有观察到变化，并且模式为lgA>>lgM>lgG。这些结果表明，尽管MRL小鼠的LPL中CD8+T细胞和γ/δTCR+细胞数量增加，但在该粘膜区室中发现浆细胞同种型(lgA>>lgM lgG)的正态分布。此外，Ipr T细胞不在胃肠道的固有层室中发育。
MRL mice, which develop a lymphoproliferative disease characterized by increased numbers of α/β T cell receptor+(TCR+) B220/6B2 + CD4−CD8−T cells [lymphoproliferation (Ipr) T cells], were studied for the effect of the Ipr/lpr mutation on the mucosal immune system in the gastrointestinal (Gl) tract. We analyzed the effect of the Ipr gene mutation on T and B cell populations in the Peyer's patches (PP) and the lamina propria lymphocytes (LPLs), as examples of major IgA inductive and effector tissues in the Gl tract respectively. Normal mouse PP contain B cells committed to IgA (surface lgA+) but only low numbers of B cells producing IgA. However, enhanced spontaneous IgA and IgG synthesis occurs in the PP of MRL mice. Further, we have now shown that PP of MRL mice are populated by Ipr T cells. Interestingly, Ipr T cells were not present in significant numbers in LPLs of MRL mice, even in older animals. Of interest was the finding that the ratio of CD4+to CD8+T cells in the lamina propria was lower in MRL when compared with control mice, and the CD8+T cell subset actually predominates in LPLs of autoimmune mice. In addition, the number of γ/δ TCR+T cells in LPL of MRL Ipr/lpr mice was significantly increased, especially in MRL Ipr/lpr mice at 6 and 12 weeks of age. When the isotype distribution of B cells in LPLs was analyzed, no changes were noted in MRL Ipr/lpr mice in comparison with MRL +/+ or normal control mice, and the pattern was lgA> >lgM >lgG. These results show that although increased numbers of CD8+T cells and γ/δ TCR + cells occur in the LPLs of MRL mice, a normal distribution of plasma cell isotypes (lgA> >lgM lgG) is found in this mucosal compartment. Further, Ipr T cells do not develop in the lamina propria compartment of the Gl tract.