What Is Stress? Dose-Response Effects in Commonly Used in Vitro Stress Assays
What Is Stress? Dose-Response Effects in Commonly Used in Vitro Stress Assays
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DOI：
10.1104/pp.113.234641
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发表时间：
2014-06-01
期刊：
影响因子：
7.4
通讯作者：
Inze, Dirk
中科院分区：
文献类型：
--
作者：
Claeys, Hannes;Van Landeghem, Sofie;Inze, Dirk
离体胁迫测定通常用于研究植物对非生物胁迫的响应和评估胁迫耐受性。文献综述表明，大多数研究使用非常高的压力水平和测量标准，如发芽，植物存活，或视觉症状的发展，如漂白。然而，我们发现，这些参数是非常严重的压力的指标，因此，这些研究只提供不完整的信息，在拟南芥（拟南芥）的压力敏感性。类似地，转录本分析揭示了典型的胁迫标记物仅在幼苗中的高胁迫水平下被诱导。因此，需要工具来研究轻度压力的影响。我们发现，常用的胁迫诱导剂甘露醇，山梨醇，氯化钠，和过氧化氢的影响芽生长在一个高度特定的和剂量依赖性的方式。因此，芽生长是一个敏感的，相关的，易于测量的表型，以评估在广泛的胁迫水平的胁迫耐受性。最后，我们的数据表明，当使用甘露醇作为兴奋剂时应小心。
In vitro stress assays are commonly used to study the responses of plants to abiotic stress and to assess stress tolerance. A literature review reveals that most studies use very high stress levels and measure criteria such as germination, plant survival, or the development of visual symptoms such as bleaching. However, we show that these parameters are indicators of very severe stress, and such studies thus only provide incomplete information about stress sensitivity in Arabidopsis (Arabidopsis thaliana). Similarly, transcript analysis revealed that typical stress markers are only induced at high stress levels in young seedlings. Therefore, tools are needed to study the effects of mild stress. We found that the commonly used stress-inducing agents mannitol, sorbitol, NaCl, and hydrogen peroxide impact shoot growth in a highly specific and dose-dependent way. Therefore, shoot growth is a sensitive, relevant, and easily measured phenotype to assess stress tolerance over a wide range of stress levels. Finally, our data suggest that care should be taken when using mannitol as an osmoticum.