Simulated data sets for single molecule kinetics: some limitations and complications of data analysis.
Simulated data sets for single molecule kinetics: some limitations and complications of data analysis.
复制标题
单分子动力学模拟数据集：数据分析的一些局限性和复杂性。
DOI：
10.1007/s00249-006-0067-5
复制
发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Steel,Duncan
中科院分区：
文献类型：
--
作者：
Shi,Jue;Gafni,Ari;Steel,Duncan
When the fluorescence intensity of a chromophore attached to or bound in an enzyme relates to a specific reactive step in the enzymatic reaction, a single molecule fluorescence study of the process reveals a time sequence in the fluorescence emission that can be analyzed to derive kinetic and mechanistic information. Reports of various experimental results and corresponding theoretical studies have provided a basis for interpreting these data and understanding the methodology. We have found it useful to parallel experiments with Monte Carlo simulations of potential models hypothesized to describe the reaction kinetics. The simulations can be adapted to include experimental limitations, such as limited data sets, and complexities such as dynamic disorder, where reaction rates appear to change over time. By using models that are known a priori, the simulations reveal some of the challenges of interpreting finite single-molecule data sets by employing various statistical signatures that have been identified.
