Porous Three-Dimensional Polyurethane Scaffolds Promote Scar-Free Endogenous Regeneration After Acute Brain Hemorrhage.
Porous Three-Dimensional Polyurethane Scaffolds Promote Scar-Free Endogenous Regeneration After Acute Brain Hemorrhage.
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DOI：
10.1007/s12975-023-01212-x
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发表时间：
2023-11
影响因子：
6.9
通讯作者：
Qiao Zhang;Jinlin Chen;Jingjing Lin;Ruichao Liang;Min He;Yanchao Wang;Hong Tan
中科院分区：
文献类型：
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作者：
Qiao Zhang;Jinlin Chen;Jingjing Lin;Ruichao Liang;Min He;Yanchao Wang;Hong Tan
脑出血（ICH）是中风最致命的亚型，与显著的发病率和死亡率相关。尽管脑出血的临床治疗取得了进展，但脑出血后内源性脑再生的进展有限。脑再生失败的主要原因是脑出血后继发性损伤引起的再生微环境的抑制。在这项研究中，我们研究了一种三维可生物降解水性聚氨酯（BWPU）支架作为促进脑出血后大脑再生的工具。在血肿清除后植入腔内，这些植入的支架可以作为储存库；储存一系列坏死碎片、细胞因子和趋化因子；并将小胶质细胞/巨噬细胞吸引到它们的毛孔中。随后，这些小胶质细胞/巨噬细胞被极化为m1样亚型以消除这些物质。这一过程分散了m1样免疫细胞，阻止了脑出血后致密胶质无瘢痕结构的形成。支架中的炎症细胞包括无疤痕分泌生长因子和细胞外基质（ECM）蛋白，并进一步诱导富集了以再生为主的m2样免疫细胞微环境，促进内源性脑再生和功能恢复。总之，在这项工作中，我们揭示了BWPU支架作为促进脑出血后内源性脑组织再生的工具的潜力和机制。
Intracerebral hemorrhage (ICH) is the most lethal subtype of stroke and is associated with significant morbidity and mortality. Despite advances in the clinical treatment of ICH, limited progress has been made regarding endogenous brain regeneration after ICH. Failure of brain regeneration is mainly attributed to the inhibitive regenerative microenvironment caused by secondary injury after ICH. In this study, we investigated a three-dimensional biodegradable waterborne polyurethane (BWPU) scaffold as a tool to promote brain regeneration after ICH. After implantation into the cavity following hematoma evacuation, these implanted scaffolds could act as a reservoir; store a series of necrotic debris, cytokines, and chemokines; and attract microglia/macrophages to their pores. Subsequently, these microglia/macrophages were polarized into the M1-like subtype to eliminate these substances. This process disperses M1-like immune cells and prevents the formation of dense glial scar-free structures after ICH. Inflammatory cells in scaffolds include scar-free secreted growth factors and extracellular matrix (ECM) proteins, and further induce a M2-like immune cells enriched regeneration-predominant microenvironment to promote endogenous brain regeneration with functional recovery. In summary, in this work, we have revealed the potential and mechanism of the BWPU scaffold as a tool to promote endogenous brain tissue regeneration after ICH.