The Propagation, Quantification, and Storage of Vesicular Stomatitis Virus.
The Propagation, Quantification, and Storage of Vesicular Stomatitis Virus.
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DOI：
10.1002/cpmc.110
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发表时间：
2020-09-01
影响因子：
--
通讯作者：
Ferran, Maureen C
中科院分区：
文献类型：
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作者：
Abdelmageed, Alaa A;Ferran, Maureen C
水泡性口炎病毒（VSV）是弹状病毒科负链单股RNA病毒的典型成员。数十年来，该病毒一直被用作强大的模型系统，目前正被用作疫苗平台和溶瘤剂。在此，我们介绍水泡性口炎病毒的繁殖、定量和储存方法。我们还回顾了处理VSV的正确安全规程，美国疾病控制与预防中心将其归类为生物安全二级病原体。© 2020 威利期刊有限责任公司。
基本方案1：水泡性口炎病毒种毒的制备、纯化与储存
基本方案2：通过空斑试验对水泡性口炎病毒进行定量
支持方案：非洲绿猴肾细胞（Vero细胞）的培养
Vesicular stomatitis virus (VSV) is the prototypical member of the Rhabdoviridae family of negative-sense single-stranded RNA viruses. This virus has been used as a powerful model system for decades and is currently being used as a vaccine platform and an oncolytic agent. Here, we present methods to propagate, quantitate, and store VSV. We also review the proper safety protocol for the handling of VSV, which is classified as a Biosafety Level 2 pathogen by the United States Centers for Disease Control and Prevention. © 2020 Wiley Periodicals LLC. Basic Protocol 1: Generation, purification, and storage of vesicular stomatitis virus stocks Basic Protocol 2: Quantification of vesicular stomatitis virus by plaque assay Support Protocol: Propagation of Vero cells.