Experimental infection of ponies with equine influenza A2 (H3N8) virus strains of different pathogenicity elicits varying interferon and interleukin-6 responses
Experimental infection of ponies with equine influenza A2 (H3N8) virus strains of different pathogenicity elicits varying interferon and interleukin-6 responses
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DOI：
10.1089/088282403763635456
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发表时间：
2003-01-01
期刊：
影响因子：
2.2
通讯作者：
Hannant, D
中科院分区：
文献类型：
--
作者：
Wattrang, E;Jessett, DM;Hannant, D
干扰素（IFN）、白细胞介素-6 （IL-6）和肿瘤坏死因子（TNF）的产生在A2流感病毒感染过程中被监测。比较了Newmarket/2/93和Sussex/89两种病毒株的效果，其中后者在临床症状方面被认为更具致病性。10匹马感染A/马/苏塞克斯/89型流感，10匹马感染A/马/纽马克特/2/93型流感。正如预期的那样，感染苏塞克斯/89的小马表现出最明显的临床症状，但与纽马克特/2/93相比，病毒排泄没有显着差异。所有感染苏塞克斯/89的小马的鼻腔分泌物中都检测到IFN，但只有2匹感染纽马克特/2/93的小马的鼻腔分泌物中检测到IFN。动物血清中未检出IFN。从第2天起，所有实验动物的鼻腔分泌物中都检测到IL-6活性，但在感染苏塞克斯/89的小马中观察到明显更高的IL-6反应。在收集的任何样本中均未检测到TNF活性。总之，马甲型h1n1流感感染在小马中引起局部，在某些情况下引起全身的IFN和IL-6反应。有趣的是，有一些证据表明，细胞因子反应的持续时间和水平可能与流感病毒株的致病性有关。
The production of interferon (IFN), interleukin-6 (IL-6), and tumor necrosis factor (TNF) was monitored in horses during the course of influenza A2 virus infections. The effects of two virus strains, Newmarket/2/93 and Sussex/89, were compared, of which the latter is considered the more pathogenic in terms of clinical signs. Ten naive ponies were infected with influenza A/equine/Sussex/89 and 10 with influenza A/equine/Newmarket/2/93, respectively. As expected ponies infected with Sussex/89 showed the most pronounced clinical signs but there was no notable difference in viral excretion compared with Newmarket/2/93. IFN was detected in nasal secretions of all ponies infected with Sussex/89 but only in 2 ponies infected with Newmarktet/2/93. IFN was not detected in serum of any animal. IL-6 activity was detected in nasal secretions of all experimental animals from day 2 and onwards, but showed markedly higher IL-6 responses were observed in ponies infected with Sussex/89. No TNF activity was detected in any of the samples collected. In summary, equine influenza A 2 infections elicited local, and in some cases systemic, IFN and IL-6 responses in the ponies. Interestingly, there was some evidence that the duration and levels of cytokine responses may be related to the pathogenicity of the influenza strains.