Automated selection of anti-protein aptamers
Automated selection of anti-protein aptamers
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DOI：
10.1016/s0968-0896(01)00028-1
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发表时间：
2001-10-01
影响因子：
3.5
通讯作者：
Ellington, AD
中科院分区：
文献类型：
--
作者：
Cox, JC;Ellington, AD
核酸结合种（适体）的体外选择通常是重复的，耗时的，并且不适合高通量应用。我们采用自动化工作站来选择抗蛋白适配体；作为一个例子，我们展示了抗溶菌酶适体的选择，作为细胞裂解的有效抑制剂。自动化带来的吞吐量的提高应该会加强适体技术在快速发展的蛋白质组学领域的应用。(C) 2001年Elsevier Science Ltd.出版
The in vitro selection of nucleic acid binding species (aptamers) is frequently repetitive, time-consuming, and poorly adapted to high-throughput applications. We have adapted automated workstations to select anti-protein aptamers; as an example, we demonstrated the selection of anti-lysozyme aptamers that function as efficient inhibitors of cell lysis. The increases in throughput brought about by automation should potentiate the application of aptamer technology to the rapidly growing field of proteomics. (C) 2001 Published by Elsevier Science Ltd.