Meningeal inflammatory response and fibrous tissue remodeling around intracortical implants: An in vivo two-photon imaging study
Meningeal inflammatory response and fibrous tissue remodeling around intracortical implants: An in vivo two-photon imaging study
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DOI：
10.1016/j.biomaterials.2018.12.031
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发表时间：
2019-03-01
期刊：
影响因子：
14
通讯作者：
Cui, X. T.
中科院分区：
文献类型：
--
作者：
Eles, J. R.;Vazquez, A. L.;Cui, X. T.
脑膜炎症和神经电极阵列的封装是设备失败的主要原因，但人们对其如何随时间发展或是什么触发因素知之甚少。为了了解神经电极植入后脑膜组织的反应，我们使用CX3CR1-GFP小鼠体内双光子显微镜对电极植入后1个月的脑膜炎性细胞行为和I型胶原重塑进行了定量研究。我们在电极植入后的第一天定义了一个迁移窗口，其特点是炎性细胞沿着脑膜中的电极迁移，以及细胞通过脑膜小静脉的运输。这种迁移窗口在植入后2天减弱，但在接下来的一个月里，脑膜胶原-I重塑以符合电极表面并增厚。这项工作表明，初始脑膜炎性细胞浸润和脑膜I型胶原重塑有明显的时间进程。这可能是植入后早期调节和减轻脑膜炎症的治疗窗口。
Meningeal inflammation and encapsulation of neural electrode arrays is a leading cause of device failure, yet little is known about how it develops over time or what triggers it. This work characterizes the dynamic changes of meningeal inflammatory cells and collagen-I in order to understand the meningeal tissue response to neural electrode implantation, We use in vivo two-photon microscopy of CX3CR1-GFP mice over the first month after electrode implantation to quantify changes in inflammatory cell behavior as well as meningeal collagen-I remodeling. We define a migratory window during the first day after electrode implantation hallmarked by robust inflammatory cell migration along electrodes in the meninges as well as cell trafficking through meningeal venules. This migratory window attenuates by 2 days post-implant, but over the next month, the meningeal collagen-I remodels to conform to the surface of the electrode and thickens. This work shows that there are distinct time courses for initial meningeal inflammatory cell infiltration and meningeal collagen-I remodeling. This may indicate a therapeutic window early after implantation for modulation and mitigation of meningeal inflammation.