Interleukin-31 does not induce immediate itch in atopic dermatitis patients and healthy controls after skin challenge
Interleukin-31 does not induce immediate itch in atopic dermatitis patients and healthy controls after skin challenge
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DOI：
10.1111/all.12316
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发表时间：
2014-01-01
期刊：
影响因子：
12.4
通讯作者：
Maurer, M.
中科院分区：
文献类型：
--
作者：
Hawro, T.;Saluja, R.;Maurer, M.
背景白细胞介素-31（IL-31）最令人感兴趣的功能是在病理条件下诱导瘙痒，如特应性皮炎（AD）。到今天为止，IL-31的这一特点是在体内测试只有在animal models.MethodsTen AD患者和10名健康对照组进行了挑战与IL-31和NaCl（阴性对照）皮肤点刺试验。对另外20名健康对照进行组胺皮肤点刺试验。瘙痒和局部炎症反应的皮肤进行了评估，长达72 h. ResultsAll组胺激发受试者发生立即瘙痒（即在第一个5 min内）。相比之下，只有一个IL-31-和两个NaCl激发的受试者报告在激发部位立即瘙痒（持续时间短，2- 6分钟）。9名受试者（5名AD患者）报告了对IL-31激发的迟发性瘙痒反应，平均延迟143分钟。无受试者报告组胺或NaCl试验的迟发性瘙痒反应。AD患者和健康志愿者IL-31诱导的瘙痒开始时间、持续时间和强度无显著差异。IL-31诱导瘙痒的迟发性支持IL-31通过角质形成细胞和次级介质间接发挥其瘙痒作用，而不是通过其在皮肤神经上的受体。
BackgroundThe most intriguing function attributed to interleukin-31 (IL-31) is its ability to induce pruritus in pathologic conditions, such as atopic dermatitis (AD). As of today, this feature of IL-31 was tested in vivo only in animal models.MethodsTen patients with AD and 10 healthy controls were challenged with IL-31 and NaCl (negative control) by skin prick testing. Twenty additional healthy controls were subjected to skin prick testing with histamine. Itch and local inflammatory responses of the skin were assessed for up to 72h.ResultsAll of the histamine-challenged subjects developed immediate pruritus (i.e. within the first 5min). In contrast, only one IL-31- and two of the NaCl-challenged subjects reported immediate itch at the provocation site (short lasting, for 2-6min). Nine subjects (five patients with AD) reported late itch responses to IL-31 challenges with a mean delay of 143min. No subject reported late itch responses to histamine or NaCl testing. There was no significant difference in IL-31-induced itch start time, duration and intensity between patients with AD and healthy volunteers.ConclusionIL-31 does not induce immediate itch responses in humans. The late onset of IL-31-induced itch supports the notion that IL-31 exerts its pruritic effect indirectly via keratinocytes and secondary mediators, rather than through its receptors on cutaneous nerves.