Left-handed DNA in restriction fragments and a recombinant plasmid
Left-handed DNA in restriction fragments and a recombinant plasmid
复制标题
限制性片段中的左手 DNA 和重组质粒
作者：
J. Klysik;S. Stirdivant;J. E. Larson;P. A. Hart;R. Wells
插入DNA限制性片段内的（dC-dG）的合成寡聚物的圆二色性和31 P-NMR表明，右手B结构可以存在于左手Z结构附近。此外，在重组质粒的小（dC-dG）片段（1.3%）中，这种盐诱导的向Z-型的转变显著影响质粒的超螺旋。这些观察结果对自然发生的相关简单序列在基因活性调节中的假定作用有影响。
Circular dichroism and 31P-NMR on synthetic oligomers of (dC-dG) inserted within DNA restriction fragments indicate that the right-handed B-structure can exist in close proximity to the left-handed Z-structure. Also, this salt-induced transition to Z-form in a small (dC-dG) segment (1.3%) of a recombinant plasmid markedly influenced the supercoil of the plasmid. These observations have implications for the postulated role of naturally occurring related simple sequences in the regulation of gene activity.
DNA 结构和基因调控。
DOI：
10.1016/s0079-6603(08)60674-1
发表时间：
1980
期刊：
Progress in nucleic acid research and molecular biology
影响因子：
--
作者：
Wells,RD;Goodman,TC;Hillen,W;Horn,GT;Klein,RD;Larson,JE;Muller,UR;Neuendorf,SK;Panayotatos,N;Stirdivant,SM
通讯作者：
Stirdivant,SM