Impact of Vitamin A Supplementation on RAR Gene Expression in Multiple Sclerosis Patients
Impact of Vitamin A Supplementation on RAR Gene Expression in Multiple Sclerosis Patients
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DOI：
10.1007/s12031-013-0090-9
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发表时间：
2013-10-01
影响因子：
3.1
通讯作者：
Saboor-Yarghi, Ali Akbar
中科院分区：
文献类型：
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作者：
Bitarafan, Sama;Harirchian, Mohammad Hossein;Saboor-Yarghi, Ali Akbar
维生素A及其衍生物已被证明通过视黄酸受体（RAR）调节免疫系统。本研究探讨补充棕榈酸视黄醇对多发性硬化症（MS）患者外周血单个核细胞（PBMCs） RAR亚型基因表达的影响。该研究设计为双盲随机临床试验，评估复发缓解型多发性硬化症患者。两组患者每2周服用5万IU维生素D3胶囊1粒，每周肌肉注射干扰素β -1a 1次。干预组每天服用一粒25,000 IU棕榈酸视黄醇胶囊，持续6个月，安慰剂组每天服用一粒安慰剂胶囊。从参与者中分离pbmc，通过实时荧光定量PCR检测rar - α和rar - γ基因的表达水平变化。补充后，干预组rar - α基因表达水平较安慰剂组显著降低（p = 0.03）；rar - γ基因表达无显著变化（p = 0.10）。这些结果表明，补充维生素A可以显著下调MS患者pbmc中rar - α基因的表达，提示维生素A对免疫系统的作用存在体内调节机制。
Vitamin A and its derivatives have been shown to modulate the immune system via retinoic acid receptor (RAR). This study explored the impact of retinyl palmitate supplementation on RAR subtype gene expression in peripheral blood mononuclear cells (PBMCs) in multiple sclerosis (MS) patients. The study designed as a double-blind randomized clinical trial in which relapsing remitting multiple sclerosis patients were evaluated. Both groups received one capsule 50,000 IU vitamin D3 per 2 weeks and one intramuscular injection interferon beta-1a per week. The intervention group received one 25,000 IU retinyl palmitate capsule daily for 6 months and the placebo group received one placebo capsule daily. The PBMCs were isolated from participants and the expression level changes of RAR-alpha and RAR-gamma genes were determined by real-time PCR. After supplementation, in the intervention group, the RAR-alpha gene expression level was significantly decreased compared to the placebo group (p = 0.03); however, the expression of RAR-gamma gene did not significantly change (p = 0.10). These results show that vitamin A supplementation can significantly downregulate the expression of RAR-alpha gene in PBMCs of MS patients that suggest the presence of in vivo regulatory mechanisms for the action of vitamin A on the immune system.