Roseovarius antarcticus sp. nov., isolated from a decayed whale bone.
Roseovarius antarcticus sp. nov., isolated from a decayed whale bone.
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DOI：
10.1099/ijs.0.000259
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发表时间：
2015-07
影响因子：
2.8
通讯作者：
Sangsang Deng;F. Jiang;Xulu Chang;Zhihao Qu;Lvzhi Ren;Yumin Zhang;Wenjing Kan;Xuyang Da;Xia Qiu;Myongchol Kim;C. Fang;Fang Peng
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文献类型：
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作者：
Sangsang Deng;F. Jiang;Xulu Chang;Zhihao Qu;Lvzhi Ren;Yumin Zhang;Wenjing Kan;Xuyang Da;Xia Qiu;Myongchol Kim;C. Fang;Fang Peng
从南极洲南设得兰群岛乔治王岛东海岸的鲸骨中分离到一株淡黄色、卵圆形至杆状的出芽细菌，命名为M-S13-148（T）菌株。菌株M-S13-148（T）表现出运动性、需氧生长，革兰氏染色阴性。菌株M-S13-148（T）的过氧化氢酶和氧化酶均为阳性。在pH 6.0-9.0、4-42 °C和0-14%（w/v）NaCl下观察到生长。新菌株含有二磷脂酰甘油，磷脂酰甘油，磷脂酰胆碱，磷脂酰乙醇胺和未知的磷脂作为主要的极性脂质。占优势的细胞脂肪酸是特征8（C18：1ω 7 c和/或C18：1ω6c）（58.8%）和C16：0（11.7%）的总和。呼吸醌为Q-10，DNA G + C含量为60.9mol%。基于16 S rRNA基因序列的邻近连接、最大似然和最小进化系统发育树表明，菌株M-S13-148（T）属于Roseovarius属，与南海玫瑰花菌（Roseovarius nanhaiticus）CCTCC AB 208317（T）的亲缘关系最近（16 S rRNA基因序列相似性为93.72%）。16 S rRNA基因序列的相似性为91.81%至93.94%。根据表型、分子和化学分类学特征，菌株M-S13-148被认为代表Roseovarius属的一个新种，本文提出模式菌株为M-S13-148（T）（= CCTCC AB 2014072（T）= LMG 28420（T））。
A pale yellow, ovoid- to rod-shaped and budding bacterium, designated strain M-S13-148(T), was isolated from a decayed bone of whale from the eastern coast of King George Island, South Shetlands, Antarctica. Strain M-S13-148(T) exhibited motility, aerobic growth and was Gram-stain-negative. Strain M-S13-148(T) was positive for catalase and oxidase. Growth was observed at pH 6.0-9.0, at 4-42 °C and with 0-14% (w/v) NaCl. The novel strain contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and an unknown phospholipid as the major polar lipids. The dominant cellular fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), (58.8%) and C16 : 0 (11.7%). The respiratory quinone was Q-10 and the DNA G + C content was 60.9 mol%. Neighbour-joining, maximum-likelihood and minimum-evolution phylogenetic trees, based on 16S rRNA gene sequences, indicated that strain M-S13-148(T) belonged to the genus Roseovarius and was most closely related to Roseovarius nanhaiticus CCTCC AB 208317(T) (93.72% 16S rRNA gene sequence similarity). The 16S rRNA gene sequence similarity with respect to members of the genus Roseovarius ranged from 91.81 to 93.94%. On the basis of phenotypic, molecular and chemotaxonomic characteristics, strain M-S13-148 is considered to represent a novel species of the genus Roseovarius, for which the name Roseovarius antarcticus sp. nov., is proposed. The type strain is M-S13-148(T) ( = CCTCC AB2014072(T) = LMG 28420(T)).