Short consensus probes with 3′-minor groove binder of the immunoglobulin heavy-chain gene for real-time quantitative PCR in B-cell non-Hodgkin lymphomas
Short consensus probes with 3′-minor groove binder of the immunoglobulin heavy-chain gene for real-time quantitative PCR in B-cell non-Hodgkin lymphomas
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DOI：
10.1038/labinvest.3700092
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发表时间：
2004-07-01
影响因子：
5
通讯作者：
Masuda, T
中科院分区：
文献类型：
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作者：
Uchiyama, M;Maesawa, C;Masuda, T
我们使用T-小沟结合物（MGB）技术开发免疫球蛋白重链（IgH）基因的共识荧光标记探针，用于检测B细胞非霍奇金淋巴瘤（B-NHL）的微小残留病（MRD）。来自59名B-NHL患者的序列数据显示，由于体细胞超突变和可变VH的使用，共有区域很窄，这表明很难设计普通的非MGB探针。MGB探针的特点是长度较短，但熔解温度较高，比普通的非MGB探针更适合这种情况。事实上，目前的数据表明，使用MGB探针（34/59，57.6%）比使用Donovan等人设计的非MGB探针（23/59，39.0%）多检测约20%的病例。MGB技术可用于设计IgH基因的共有荧光标记探针以检测MRD。
We used T-minor groove binder (MGB) technology to develop consensus fluorogenically labeled probes of the immunoglobulin heavy-chain (IgH) gene for detecting minimal residual disease (MRD) in B-cell non-Hodgkin lymphoma (B-NHL). Sequence data from 59 patients with B-NHLs revealed a narrow consensus region as a result of somatic hypermutations and variable VH usage, indicating that it would be difficult to design ordinary non-MGB probes. MGB probes, characterized by shorter length but higher melting temperature, are more suitable for this situation than ordinary non-MGB probes. In fact, the present data indicated that about 20% more cases were detectable with MGB probes (34/59, 57.6%) than with the non-MGB probes (23/59, 39.0%) designed by Donovan et al. MGB technology is useful for the design of consensus fluorogenically labeled probes of the IgH gene for detecting MRD.