Biocompatibility Assessment of PLCL-Sericin Copolymer Membranes Using Wharton's Jelly Mesenchymal Stem Cells.
Biocompatibility Assessment of PLCL-Sericin Copolymer Membranes Using Wharton's Jelly Mesenchymal Stem Cells.
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DOI：
10.1155/2016/5309484
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发表时间：
2016
影响因子：
4.3
通讯作者：
Wongkham W
中科院分区：
文献类型：
--
作者：
Inthanon K;Daranarong D;Techaikool P;Punyodom W;Khaniyao V;Bernstein AM;Wongkham W
基于干细胞的组织工程需要生物相容性材料，使细胞能够大规模粘附、扩张和分化。临床应用的理想生物材料应不含引起免疫反应和病原体感染的哺乳动物产品。我们发明了一种通过静电纺丝制备的新型可生物降解的聚（L-乳酸-共-ε-己内酯）-丝胶（PLCL-SC）共聚物膜。浓度为 2.5 或 5% (w/v) SC 的膜表现出合格的质地特征和非细胞毒性释放曲线。该膜的亲水性能比标准 PLCL 和商用聚苯乙烯 (PS) 高出 35-40%。膜特性的改善归因于在其表面添加了新的功能性酰胺基团：C=O、N-H 和 C-N。膜的降解是可控的，具体取决于SC的含量比例。热分析结果表明膜具有优异的稳定性和结晶度。这些膜通过增加细胞周期蛋白 A 增强人沃顿胶间充质干细胞 (hWJMSC) 增殖，并通过上调粘着斑激酶 (FAK) 促进细胞粘附。在膜上，hWJMSC随着巢蛋白的出现分化成神经元谱系。这些数据表明 PLCL-SC 电纺膜具有一些可用于组织工程和医疗应用的特性。
Stem cells based tissue engineering requires biocompatible materials, which allow the cells to adhere, expand, and differentiate in a large scale. An ideal biomaterial for clinical application should be free from mammalian products which cause immune reactivities and pathogen infections. We invented a novel biodegradable poly(L-lactic-co-ε-caprolactone)-sericin (PLCL-SC) copolymer membrane which was fabricated by electrospinning. Membranes with concentrations of 2.5 or 5% (w/v) SC exhibited qualified texture characteristics with a noncytotoxic release profile. The hydrophilic properties of the membranes were 35–40% higher than those of a standard PLCL and commercial polystyrene (PS). The improved characteristics of the membranes were due to an addition of new functional amide groups, C=O, N–H, and C–N, onto their surfaces. Degradation of the membranes was controllable, depending on the content proportion of SC. Results of thermogram indicated the superior stability and crystallinity of the membranes. These membranes enhanced human Wharton's jelly mesenchymal stem cells (hWJMSC) proliferation by increasing cyclin A and also promoted cell adhesion by upregulating focal adhesion kinase (FAK). On the membranes, hWJMSC differentiated into a neuronal lineage with the occurrence of nestin. These data suggest that PLCL-SC electrospun membrane represents some properties which will be useful for tissue engineering and medical applications.