Growth hormone-releasing factor from ovine and caprine hypothalamus: isolation, sequence analysis and total synthesis.
Growth hormone-releasing factor from ovine and caprine hypothalamus: isolation, sequence analysis and total synthesis.
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来自绵羊和山羊下丘脑的生长激素释放因子：分离、序列分析和全合成。
DOI：
10.1016/0006-291x(84)90582-5
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发表时间：
1984
影响因子：
3.1
通讯作者：
Guillemin,R
中科院分区：
文献类型：
--
作者：
Brazeau,P;Böhlen,P;Esch,F;Ling,N;Wehrenberg,WB;Guillemin,R
采用酸提取、免疫亲和层析、凝胶过滤、反相高效液相色谱等方法，从2100只绵羊和2600只山羊下丘脑中分离出具有高内源性生长激素释放活性的多肽(GRF)。用气-液相测序和肽谱对44个氨基酸的羊多肽进行结构表征，确定其一级结构为Tyr-Ala-Asp-Ala-Ile-Phe-Thr-Asn-Ser-Tyr-Arg-Lys-Ile-Leu-Gly-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Met-Asn-Arg-Gln-Gln-Gly-Glu-Arg-Asn-Gln-Glu-Gln-Gly-Ala-Lys-Val-Arg-Leu-NH2.山羊GRF具有相同的序列，除了第13位的异亮氨酸残基被Valine取代外，因此与牛GRF相同。
Peptides with high intrinsic growth hormone releasing activity (growth hormone-releasing factor, GRF) were isolated from 2100 ovine and 2600 caprine (goat) hypothalami by means of acid extraction, immunoaffinity chromatography, gel filtration and reverse phase HPLC. Structural characterization of the 44 amino acid ovine peptide by gas-liquid phase sequencing and peptide mapping established its primary structure as Tyr-Ala-Asp-Ala-Ile-Phe-Thr-Asn-Ser-Tyr-Arg-Lys-Ile-Leu-Gly-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile- Met-Asn-Arg-Gln-Gln-Gly-Glu-Arg-Asn-Gln-Glu-Gln-Gly-Ala-Lys-Val-Arg-Leu-NH2. Caprine GRF was found to possess the same sequence except for the replacement of the isoleucine residue in position 13 with valine and thus is identical to bovine GRF.