Rapid, Affordable and Portable Medium-Throughput Molecular Device for Zika Virus
Rapid, Affordable and Portable Medium-Throughput Molecular Device for Zika Virus
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DOI：
10.1038/srep38223
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发表时间：
2016-12-09
影响因子：
4.6
通讯作者：
Wong, Season
中科院分区：
文献类型：
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作者：
Chan, Kamfai;Weaver, Scott C.;Wong, Season
寨卡病毒（ZIKV）作为胎儿小头畸形和格林-巴利综合征的病原体已引起全球关注。现有的基于免疫的快速检测往往无法区分寨卡病毒和相关的黄病毒，这些病毒在中美洲和南美洲以及加勒比地区的受影响地区很常见。美国CDC和合格的州卫生部门实验室可以使用高度复杂的仪器进行逆转录聚合酶链反应（RT-PCR）ZIKV测试，周转时间长。本文报道了用于ZIKV感染的便携式和低成本分子诊断系统的初步结果。在不到15分钟的时间内，这个低成本的平台可以同时从多达12个ZIKV加标的尿液样本中自动进行高质量的RNA提取。它还可以进行逆转录重组酶聚合酶扩增反应（RT-RPA），
Zika virus (ZIKV) has gained global attention as an etiologic agent of fetal microcephaly and Guillain-Barre syndrome. Existing immuno-based rapid tests often fail to distinguish between Zika and related flaviviruses that are common in affected regions of Central and South Americas and the Caribbean. The US CDC and qualified state health department laboratories can perform the reverse transcription polymerase chain reaction (RT-PCR) ZIKV test using highly sophisticated instruments with long turnaround times. The preliminary results of a portable and low-cost molecular diagnostics system for ZIKV infection are reported here. In less than 15 minutes, this low-cost platform can automatically perform high quality RNA extraction from up to 12 ZIKV-spiked urine samples simultaneously. It can also perform reverse transcription recombinase polymerase amplification reaction (RT-RPA) in