Extreme longevity is associated with increased resistance to oxidative stress in Arctica islandica, the longest-living non-colonial animal.
Extreme longevity is associated with increased resistance to oxidative stress in Arctica islandica, the longest-living non-colonial animal.
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DOI：
10.1093/gerona/glr044
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发表时间：
2011-07
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通讯作者：
Z. Ungvari;I. Ridgway;E. Philipp;Courtney M. Campbell;P. Mcquary;Tracy T. Chow;Miguel Coelho;E. Didier;Sara Gelino;Marissa A. Holmbeck;Insil Kim;E. Levy;D. Sosnowska;W. Sonntag;S. Austad;A. Csiszar
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作者：
Z. Ungvari;I. Ridgway;E. Philipp;Courtney M. Campbell;P. Mcquary;Tracy T. Chow;Miguel Coelho;E. Didier;Sara Gelino;Marissa A. Holmbeck;Insil Kim;E. Levy;D. Sosnowska;W. Sonntag;S. Austad;A. Csiszar
我们评估了活性氧簇的产生和对氧化应激的抵抗力是否可能与海洋方蛤Arctica islandica表现出的非凡寿命有因果关系。我们通过比较活性氧的产生，抗氧化应激，抗氧化防御和蛋白质损伤消除过程中的长寿A islandica与寿命较短的硬壳蛤，Mercenaria corpusaria来验证这一假设。我们比较了基线生化概况，年龄相关的变化，并暴露于氧化应激叔丁基过氧化氢（TBHP）的反应。我们的数据支持的前提下，在一个islandica的极端长寿与衰减的细胞活性氧的产生。与M islandica鳃组织相比，减少蛋白质羰基浓度的观察表明，减少活性氧在长寿的双壳类动物的生产与较低水平的积累的大分子损伤，这表明细胞的氧化还原稳态可能决定寿命。在生物体水平上对衰老的抵抗力通常反映在细胞水平上对氧化应激的抵抗力。TBHP暴露后，我们观察到不仅长寿和抗氧化应激诱导的死亡率之间的关联，但也显着的抗氧化应激诱导的细胞死亡在较长的寿命双壳类。相反的氧化应激假说的一些期望，我们观察到，A islandica既没有表现出更大的抗氧化能力，也没有比在M mangiaria的具体活动，也没有更明显的稳态抗氧化反应TBHP曝光后。该研究也未能为A islandica的异常长寿与增强的蛋白质循环有关提供支持。我们的研究结果表明，长寿和抗氧化应激诱导的细胞死亡之间的关联在A islandica，与衰老的氧化应激假说一致，并提供了理由，详细评估的途径，涉及修复自由基介导的大分子损伤和调节细胞凋亡在世界上最长寿的非殖民动物。
We assess whether reactive oxygen species production and resistance to oxidative stress might be causally involved in the exceptional longevity exhibited by the ocean quahog Arctica islandica. We tested this hypothesis by comparing reactive oxygen species production, resistance to oxidative stress, antioxidant defenses, and protein damage elimination processes in long-lived A islandica with the shorter-lived hard clam, Mercenaria mercenaria. We compared baseline biochemical profiles, age-related changes, and responses to exposure to the oxidative stressor tert-butyl hydroperoxide (TBHP). Our data support the premise that extreme longevity in A islandica is associated with an attenuated cellular reactive oxygen species production. The observation of reduced protein carbonyl concentration in A islandica gill tissue compared with M mercenaria suggests that reduced reactive oxygen species production in long-living bivalves is associated with lower levels of accumulated macromolecular damage, suggesting cellular redox homeostasis may determine life span. Resistance to aging at the organismal level is often reflected in resistance to oxidative stressors at the cellular level. Following TBHP exposure, we observed not only an association between longevity and resistance to oxidative stress-induced mortality but also marked resistance to oxidative stress-induced cell death in the longer-living bivalves. Contrary to some expectations from the oxidative stress hypothesis, we observed that A islandica exhibited neither greater antioxidant capacities nor specific activities than in M mercenaria nor a more pronounced homeostatic antioxidant response following TBHP exposure. The study also failed to provide support for the exceptional longevity of A islandica being associated with enhanced protein recycling. Our findings demonstrate an association between longevity and resistance to oxidative stress-induced cell death in A islandica, consistent with the oxidative stress hypothesis of aging and provide justification for detailed evaluation of pathways involving repair of free radical-mediated macromolecular damage and regulation of apoptosis in the world's longest-living non-colonial animal.