A developmentally plastic adult mouse kidney cell line spontaneously generates multiple adult kidney structures.
A developmentally plastic adult mouse kidney cell line spontaneously generates multiple adult kidney structures.
复制标题
发育可塑性成年小鼠肾细胞系自发产生多个成年肾脏结构。
DOI：
10.1016/j.bbrc.2015.06.130
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发表时间：
2015
影响因子：
3.1
通讯作者：
Obara,Tomoko
中科院分区：
文献类型：
--
作者：
Webb,CarolF;Ratliff,MichelleL;Powell,Rebecca;Wirsig-Wiechmann,CelesteR;Lakiza,Olga;Obara,Tomoko
尽管诱导多能干细胞的能力为再生治疗带来了令人兴奋的新可能性，但三维肾脏的重现用于修复或替换一直是不可能的。ARID3a缺陷的小鼠组织产生了多潜能的发育可塑性细胞。因此，我们评估了表达肾脏祖细胞表面标志的成年小鼠ARID3a−/−肾脏细胞系KKPS5作为模拟肾脏发育的替代细胞来源。值得注意的是，这些细胞在体外自发发育成多细胞肾单位样结构树脂，并植入免疫受损的青竹中肾单位，在那里它们形成了小鼠肾单位结构。这些数据表明KKPS5细胞是研究肾脏发育的一个新的模型系统。
Despite exciting new possibilities for regenerative therapy posed by the ability to induce pluripotent stem cells, recapitulation of three-dimensional kidneys for repair or replacement has not been possible.ARID3a-deficient mouse tissues generated multipotent, developmentally plastic cells. Therefore, we assessed the adult mouseARID3a−/−kidney cell line, KKPS5, which expresses renal progenitor surface markers as an alternative cell source for modeling kidney development. Remarkably, these cells spontaneously developed into multicellular nephron-like structuresin vitro, and engrafted into immunocompromised medaka mesonephros, where they formed mouse nephron structures. These data implicate KKPS5 cells as a new model system for studying kidney development.