Relationship between HLA-DP gene polymorphisms and clearance of chronic hepatitis B virus infections: case-control study and meta-analysis.
Relationship between HLA-DP gene polymorphisms and clearance of chronic hepatitis B virus infections: case-control study and meta-analysis.
复制标题
DOI：
10.1016/j.meegid.2012.03.026
复制
发表时间：
2012-08
期刊：
影响因子：
--
通讯作者：
Ze-hui Yan;Shun Tan;Y. Dan;Xiaowen Sun;Guohong Deng;Yuming Wang
中科院分区：
文献类型：
--
作者：
Ze-hui Yan;Shun Tan;Y. Dan;Xiaowen Sun;Guohong Deng;Yuming Wang
乙肝病毒感染是世界范围内一个严重的公共卫生问题。人类白细胞抗原DP(HLADP)的两个常见基因变异(rs3077和rs9277535)已被报道与日本和泰国人群中持续的乙肝病毒感染有关。为了证实这种关联是否可以在中国人群中重复，我们进行了一项独立的病例对照研究，用TaqMan SNP基因分型方法对282例慢性乙肝病毒携带者和自然恢复携带者的两个基因多态性(rs3077和rs9277535)进行了基因分型。为了提供更明确的结论，本文采用随机效应模型，运用德西蒙尼和莱尔德的方法，对五项研究进行了荟萃分析。通过对年龄、性别、饮酒等协变量进行Logistic回归分析，独立病例对照研究结果显示，rs3077和rs9277535的等位基因纯合子(AA)与慢性乙肝持续感染的危险性/保护性降低显著相关(rs3077：P=0.0017，OR=0.29，95%CI=0.13~0.62；rs9277535，P=0.0004，OR=0.26，95%CI=0.12~0.54)。Meta分析结果还显示，rs3077-A和rs9277535-A等位基因对乙肝病毒感染的清除率增加(rs3077：OR=0.57，95%CI=0.44~0.75；rs9277535：OR=0.56，95%CI=0.47~0.63)。这些结果进一步证实了人类白细胞抗原-DP基因变异对持续感染的乙肝病毒自发清除风险有很强的影响。在中国汉族人群中，A等位基因rs3077和rs9277535对持续乙肝病毒感染的自发清除具有较强的保护作用。
Hepatitis B virus (HBV) infection is a serious public health problem worldwide. Two common genetic variants (rs3077 and rs9277535) of human leukocyte antigen DP (HLA-DP) have been reported to be associated with persistent HBV infection in populations of Japan and Thailand. To confirm whether the association can be replicated in Chinese populations, an independent case–control study were conducted, and two polymorphisms (rs3077 and rs9277535) were genotyped using the TaqMan SNP genotyping assay in 282 persistent chronic HBV carriers and 64 spontaneously HBV recovered carriers. To provide a more definitive conclusion, a meta-analysis combining and summarizing five studies was performed by random-effects model using the DerSimonian and Laird’s method. By using logistic regression analysis with adjustment for covariates, including age, sex, and alcohol consumption, the results of our independent case–control study showed that the minor allele’s homozygote (AA genotype) of rs3077 and rs9277535 was significantly associated with decreasing risk/protection of HBV persistent chronic infection (for rs3077: P=0.0017, OR=0.29, 95% CI=0.13–0.62; for rs9277535, P=0.0004, OR=0.26, 95% CI=0.12–0.54). The results of meta-analysis pooling all eligible studies also showed that rs3077-A and rs9277535-A alleles were associated with an increased clearance rate to HBV infection (rs3077: OR=0.57, 95% CI=0.44–0.75; rs9277535: OR=0.56, 95% CI=0.47–0.63). These results further confirmed the strong influence of HLA-DP gene variants on risk of spontaneous HBV clearance from persistent HBV infection. Both A alleles of HLA-DP SNP rs3077 and rs9277535 showed strong protective effects for spontaneous HBV clearance from persistent HBV infection in the Han Chinese population.