Interactions between Irrigants Commonly Used in Endodontic Practice: A Chemical Analysis
Interactions between Irrigants Commonly Used in Endodontic Practice: A Chemical Analysis
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DOI：
10.1016/j.joen.2012.11.050
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发表时间：
2013-04-01
影响因子：
4.2
通讯作者：
Gomes, Brenda P. F. A.
中科院分区：
文献类型：
--
作者：
Prado, Maira;Santos, Helvecio M., Jr.;Gomes, Brenda P. F. A.
简介：这项工作的目的是通过电喷雾电离四极杆飞行时间质谱分析，在牙髓实践中最常用的冲洗剂之间的协会形成的副产品的特征。研究方法：次氯酸钠（NaOCl）（0.16%、1%、2.5%和5.25%）与2%氯己定（CHX）溶液和凝胶、17% EDTA、10%柠檬酸、37%磷酸、生理盐水溶液、乙醇和蒸馏水联合使用。CHX溶液和凝胶也与上述所有冲洗液相关。将溶液以1：1的比例混合，并使用电喷雾电离四极杆飞行时间质谱来表征形成的沉淀物。结果如下：当与1%-5.25%的NaOCl缔合时，CHX产生橙棕色沉淀，当与0.16%的NaOCl缔合时，CHX产生橙白色沉淀。当与EDTA结合时，CHX产生白色乳状沉淀，当与盐水溶液和乙醇结合时，产生盐沉淀。当CHX与柠檬酸、磷酸或蒸馏水结合时，未观察到沉淀。在NaOCl协会，沉淀发生时，只有CHX是本。结论：在CHX和NaOCl之间的缔合中观察到的橙棕色沉淀物是由于NaOCl的存在而发生的，NaOCl是一种氧化剂，导致CHX的胍基氮氯化。CHX与EDTA、生理盐水和乙醇反应生成的沉淀分别与酸碱反应、盐析过程和较低的溶解度有关。NaOCl与EDTA、柠檬酸和磷酸结合主要导致氯气形成。用蒸馏水进行中间冲洗似乎适合于防止或至少减少副产物的形成。
Introduction: The aim of this work was to characterize the by-products formed in the associations between the most commonly used irrigants in endodontic practice through electrospray ionization quadrupole time-of-flight mass spectrometry analyses. Methods: Sodium hypochlorite (NaOCl) (0.16%, 1%, 2.5%, and 5.25%) was associated with 2% chlorhexidine (CHX) solution and gel, 17% EDTA, 10% citric acid, 37% phosphoric acid, saline solution, ethanol, and distilled water. CHX solution and gel were also associated with all above mentioned irrigants. The solutions were mixed in a 1:1 ratio, and electrospray ionization quadrupole time-of-flight mass spectrometry was used to characterize the precipitates when formed. Results: CHX produced an orange-brown precipitate when associated with NaOCl from 1%-5.25% and an orange-white precipitate when associated with 0.16% NaOCl. When associated with EDTA, CHX produced a white milky precipitate, and when associated with saline solution and ethanol, a salt precipitation was produced. No precipitation was observed when CHX was associated with citric acid, phosphoric acid, or distilled water. In the NaOCl associations, precipitation occurred only when CHX was present. Conclusion: The orange-brown precipitate observed in the association between CHX and NaOCl occurs because of the presence of NaOCl, an oxidizing agent causing chlorination of the guanidino nitrogens of the CHX. The precipitates formed in the reaction of CHX with EDTA, saline solution, and ethanol were associated with acid-base reactions, salting-out process, and lower solubility, respectively. NaOCl associated with EDTA, citric acid, and phosphoric acid leads mainly to chlorine gas formation. Intermediate flushes with distilled water seem to be appropriate to prevent or at least reduce formation of by-products.