Preparation of a novel hydrophobic interaction chromatography with higher selectivity by introducing spatial recognition
Preparation of a novel hydrophobic interaction chromatography with higher selectivity by introducing spatial recognition
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通过引入空间识别制备具有更高选择性的新型疏水相互作用色谱
DOI：
10.1002/jctb.6406
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发表时间：
2020
影响因子：
3.4
通讯作者：
De‐Man Han
中科院分区：
文献类型：
--
作者：
Wei Shi;Si‐Qi Zhang;Kai‐Bin Li;Ye‐Jing Deng;Chen‐Yi Wang;Yi‐Ting Lu;Jiao‐Jiao Liu;De‐Man Han
背景疏水作用色谱（HIC）作为一种典型的分离技术，由于其作用模式单一，选择性低。本文合成了一种新型的具有印迹层的HIC树脂（Octyl@BSA），并将其用于蛋白质的吸附。牛血清白蛋白（BSA）和牛血清γ-球蛋白（bIgG）在本研究中用作模型竞争蛋白。研究了pH值、盐种类、浓度和质量比等因素对吸附的影响。静态和动态吸附实验数据表明，空间识别的引入有效地减少了竞争蛋白的吸附，从而显著提高了选择性。与原始HIC树脂相比，Octyl@BSA保留了对BSA的高结合能力，但通过空间识别拒绝其他蛋白。该树脂的最大选择性因子（α）可接近5。吸附动力学和前沿吸附实验进一步证实了在间歇和柱模式下选择性的改善。此外，Octyl@BSA的可回收性进行了测试，使用10个连续的BSA吸附-解吸cycle.CONCLUSIONThis研究表明，空间识别的引入是一个有效的策略，提高竞争吸附过程中的优势，目标蛋白质，从而提高HIC的选择性。© 2020化学工业学会
BACKGROUNDHydrophobic interaction chromatography (HIC), as a typical separation technology, often suffers from low selectivity because it has a single interaction mode. Herein, a novel HIC resin (Octyl@BSA) with imprinted layer was synthesized and used for protein adsorption. Bovine serum albumin (BSA) and bovine serum γ‐globulin (bIgG) were used as model competitive proteins in this study. The effects of pH, salt type, concentration, and mass ratio were then investigated.RESULTSThe data from the static and dynamic adsorption experiments indicated that the introduction of spatial recognition effectively reduced the adsorption of competitive proteins, leading to significant improvements in selectivity. In comparison with raw HIC resin, Octyl@BSA retained a high binding capacity for BSA but rejected other proteins through spatial recognition. The maximum selectivity factors (α) of this resin may be close to 5. The adsorption kinetics and frontal adsorption experiments further confirmed the improvement in selectivity in batch and column mode. In addition, the recyclability of Octyl@BSA was tested using ten continuous BSA adsorption–desorption cycles.CONCLUSIONThis study demonstrated that the introduction of spatial recognition was an effective strategy for enhancing the advantage of target proteins during competitive adsorption, leading to an improvement in selectivity for HIC. © 2020 Society of Chemical Industry