EFFECTS OF GRANULOCYTE COLONY-STIMULATING FACTOR ON THE PROLIFERATION AND CELL-FATE SPECIFICATION OF NEURAL STEM CELLS
EFFECTS OF GRANULOCYTE COLONY-STIMULATING FACTOR ON THE PROLIFERATION AND CELL-FATE SPECIFICATION OF NEURAL STEM CELLS
复制标题
粒细胞集落刺激因子对神经干细胞增殖和细胞命运规范的影响
DOI：
10.1016/j.neuroscience.2009.09.045
复制
发表时间：
2009-12-29
期刊：
影响因子：
3.3
通讯作者：
Hao, A.
中科院分区：
文献类型：
--
作者：
Liu, H.;Jia, D.;Hao, A.
粒细胞集落刺激因子（G-CSF）是一种调节造血祖细胞增殖、分化和存活的生长因子。越来越多的证据表明，G-CSF在不同的神经系统疾病中发挥强大的神经保护作用。然而，G-CSF是否对神经干细胞（NSCs）有直接作用仍有待阐明。本研究表明，G-CSF在体外可刺激神经干细胞增殖并促进其分化。此外，我们已经表明，G-CSF诱导的神经干细胞增殖与STAT 3的磷酸化有关，分化与分化相关基因的表达改变有关。值得注意的是，G-CSF不能启动神经干细胞的分化。G-CSF对神经干细胞增殖和分化的调节作用为设计新的干细胞治疗策略以促进脑修复提供了有益的参考。(C)2009年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Granulocyte-colony stimulating factor (G-CSF) is a growth factor that regulates proliferation, differentiation and survival of hematopoietic progenitor cells. There is growing evidence to suggest that G-CSF exerts a powerful neuroprotective effect in different neurological disorders. However, it has remained to be elucidated if G-CSF has a direct effect on neural stem cells (NSCs). Here, we show that G-CSF could stimulate the proliferation of NSCs and promote their differentiation in vitro. Additionally, we have shown that G-CSF-induced proliferation of NSCs is associated with phosphorylation of STAT3, and the differentiation is linked to altered expression of differentiation-related genes. Remarkably, G-CSF could not initiate the differentiation of NSCs. The added roles of G-CSF in regulating proliferation and differentiation of NSCs as shown in this study would serve as a useful reference in designing new stem cell therapy strategies for promoting brain recovery and repair. (C) 2009 IBRO. Published by Elsevier Ltd. All rights reserved.