Simple Surface Treatment of Cell-Culture Scaffolds with Ultrafine Bubble Water
Simple Surface Treatment of Cell-Culture Scaffolds with Ultrafine Bubble Water
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DOI：
10.1021/la5035883
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发表时间：
2014-12-23
期刊：
影响因子：
3.9
通讯作者：
Tanaka, Keiji
中科院分区：
文献类型：
--
作者：
Matsuno, Hisao;Ohta, Toshiyuki;Tanaka, Keiji
我们提出了一种新的方法来处理聚合物支架表面的细胞培养与水含有纳米气泡，称为超细气泡（UFB），具有典型的直径小于1 μ m。将在固体基底上制备的聚苯乙烯（PS）薄膜在室温下暴露于UFB水中2天。通过X射线光电子能谱（XPS），在水中的静态接触角测量，和原子力显微镜（AFM）的PS表面进行了表征。处理后的PS膜的表面化学组成和润湿性保持不变，因此PS在膜表面的聚集状态保持不变UFB水。另一方面，处理后，许多UFB吸附在疏水PS表面。为了研究UFB对支架性能的影响，检查了纤连蛋白的吸附行为，纤连蛋白是参与细胞粘附和增殖的典型细胞外基质蛋白。虽然对吸附的影响尚不清楚，但UFB处理后增强了纤连蛋白的结构变性，从而促进了PS表面成纤维细胞的增殖。
We propose a novel method to treat polymeric scaffold surfaces for cell culture with water containing nanobubbles, called ultrafine bubbles (UFBs), with typical diameters less than 1 mu m. A thin film of polystyrene (PS) prepared on a solid substrate was exposed to UFB water for 2 days at room temperature. The PS surface was characterized by X-ray photoelectron spectroscopy (XPS), static contact angle measurements in water, and atomic force microscopy (AFM). The surface chemical composition and wettability of PS films remained unchanged after treatment, so that aggregation states of PS at film surfaces remained unaltered by UFB water. On the other hand, after treatment, many UFBs were adsorbed on hydrophobic PS surfaces. To study the effect of UFBs on scaffold properties, the adsorption behavior of fibronectin, which is a typical extracellular matrix protein involved in cell adhesion and proliferation, was examined. While the effect on the adsorption was unclear, the structural denaturation of fibronectin was enhanced after UFB treatment, so that the proliferation of fibroblast cells on PS surfaces was promoted.